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| 高效液相色谱测定广西生鲜乳中的维生素A | |
| 引用本文： | 段玉林,苏骏,吴雪,张少梅,温韬,梁梦宇,陈小聪,蒙泳,周曼,宗玉英.高效液相色谱测定广西生鲜乳中的维生素A[J].中国乳品工业,2012,40(5):58-60. |
| 作者姓名： | 段玉林 苏骏 吴雪 张少梅 温韬 梁梦宇 陈小聪 蒙泳 周曼 宗玉英 |
| 作者单位： | 1. 广西产品质量监督检验院,南宁,530022 2. 广西壮族自治区环境监测中心站,南宁,530022 3. 广西壮族自治区质量技术监督局,南宁,530022 |
| 摘 要： | 为了建立生鲜乳中维生素A的一种快速、准确、灵敏度高的检测方法,样品经皂化后,经石油醚提取,浓缩后采用高效液相色谱法,以Shim-pack VP-ODS柱(4.6 mm×150 mm,5μm),柱温35℃,检测波长325 nm,流动相为甲醇∶水(98∶2,体积比),流速1.0 mL/min的条件进行实验。结果维生素A在0.174～87.2μg/mL范围内线性关系良好,相关系数r=0.9999,平均回收率为98.17%,RSD=1.90%。建立的方法准确、可靠,可以用于生鲜乳中维生素A的含量检测。 |
| 关 键 词： | 生鲜乳 维生素A 高效液相色谱 |
Detection of Vitamin A in the Gungxi fresh milk by performance liquid chromatography |
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| DUAN Yu-lin , SU Jun , WU Xue , ZHANG Shao-mei , WEN Tao , LIANG Meng-yu , CHEN Xiao-cong , MENG Yong , ZHOU Man , ZONG Yu-ying.Detection of Vitamin A in the Gungxi fresh milk by performance liquid chromatography[J].China Dairy Industry,2012,40(5):58-60. | |
| Authors: | DUAN Yu-lin SU Jun WU Xue ZHANG Shao-mei WEN Tao LIANG Meng-yu CHEN Xiao-cong MENG Yong ZHOU Man ZONG Yu-ying |
| Affiliation: | 1(1.Guanxi Product Quality Supervision and Testing Institute,Nanning 530022,China;2.Guangxi Bureau of Quality and Technical Supervision,Nanning 530022,China;3.Guangxi Environmental Protection Monitoring Central Station,Nanning 530022,China) |
| Abstract: | To establish a quick,accurate,sensitive method for analyzing Vitamin A illegally added into the fresh milk.The sample was determined after being saponified,abstracted with petroleum ether and concentrated,performance liquid chromatography was used.The column was Shim-pack VP-ODS 4.6 ×150 mm,column temperature was 35 ℃ and the mobile phase was methanol:water = 98:2(v:v) with flow rate 1.0 mL/min and the detective wavelength was 325 nm..The linear range of the calibrati on curve of Vitamin A were 0.174～87.2 μg/mL(r=0.9999).The mean rates of recovery was 98.17% and the relative standard deviations(n=5) was 1.90%.The method is accurate,realiable that can be used to determine Vitamin A in the fresh milk. |
| Keywords: | fresh milk Vitamin A performance liquid chromatography |
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