| 宇佐美曲霉酸性蛋白酶发酵工艺研究 |
| |
| 引用本文： | 李永泉,贺筱蓉.宇佐美曲霉酸性蛋白酶发酵工艺研究[J].化学反应工程与工艺,1998,14(4):382-387. |
| |
| 作者姓名： | 李永泉 贺筱蓉 |
| |
| 作者单位： | 浙江大学（西溪校区）生命科学学院 |
| |
| 摘 要： | 宇佐美曲霉Ｌ３３５是一株分泌高单位酸性蛋白酶的产生菌，本研究采用单因素搜索和正交试验，通过摇瓶发酵和２Ｌ搅拌罐发酵，对其发酵工艺进行优化。结果表明，较适宜的发酵培养基（ｇ／１００ｍＬ）为黄豆粉５．０、玉米粉１．２、鱼粉０．８、ＣａＣｌ２ ０．５、Ｎａ２ＨＰＯ４ ０．２、ＮＨ４Ｃｌ１．０、蚕蛹水解液１０．０；通气量控制２５ｈ前为１：０．４ｖ／ｖ／ｍ、２５－５０ｈ１：１．０ｖ／ｖ／ｍ，５０ｈ后１：３ｖ
|
| 关 键 词： | 宇佐美曲酶L336 发酵工艺 优化 酸性蛋白酶 |
STUDIES ON THE ACID PROTEASE FERMENTATION TECHNIQUE OF USAMII L336 |
| |
| Li Yongquan,He Xiaorong,Wen Xinhua,Hu Huacui,Lin Manxi,Pan Bo.STUDIES ON THE ACID PROTEASE FERMENTATION TECHNIQUE OF USAMII L336[J].Chemical Reaction Engineering and Technology,1998,14(4):382-387. |
| |
| Authors: | Li Yongquan He Xiaorong Wen Xinhua Hu Huacui Lin Manxi Pan Bo |
| |
| Abstract: | Asp. usamii L336 strain could produce high yield of acid protease. For optimizing its fermentation conditions, a single factor searching and orthogonal test were carried out in flask and 2L agitation fermentor. The experiment results showed that ezyme activity of acid protease was over 6100 u/mL under the optimum condition. This fermentation unit is in the lead level in China. |
| |
| Keywords: | A Usamii L336 Acid protease Fermentation technique Optimization |
| 本文献已被 CNKI 维普 等数据库收录！ |
