| 食品中3种致病李氏菌MPCR-DHPLC检测方法的建立 |
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| 引用本文： | 王耀,曹际娟,闫平平,曹远银.食品中3种致病李氏菌MPCR-DHPLC检测方法的建立[J].食品科学,2010,31(8):158. |
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| 作者姓名： | 王耀 曹际娟 闫平平 曹远银 |
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| 作者单位： | 沈阳农业大学植物免疫研究所;辽宁出入境检验检疫局;辽宁师范大学生命科学学院; |
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| 基金项目： | “十一五”国家科技支撑计划项目(2006BAK02A13) |
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| 摘 要： | 应用复合PCR(multiplex PCR,MPCR)结合变性高效液相色谱(denaturing high-performance liquid chromatography,DHPLC)技术建立食品中单核细胞增生李斯特菌、绵羊李斯特菌和英诺克李斯特菌的快速高通量检测方法。根据单核细胞增生李斯特菌、绵羊李斯特菌和英诺克李斯特菌的特异基因序列分别设计引物,MPCR扩增的产物经DHPLC技术进行快速检测。以94株参考菌株做特异性实验,并开展了重现性检测实验。MPCR-DHPLC方法同步检测到单核细胞增生李斯特菌、绵羊李斯特菌和英诺克李斯特菌的特异性阳性吸收峰,未检测到李斯特菌属其他近源种和非近源种参考菌株的阳性吸收峰,且重现性良好。该方法具有很好的特异性,可以快速、准确、高通量地检测食品中单核细胞增生李斯特菌、绵羊李斯特菌和英诺克李斯特菌,是食品微生物快速检测的新技术。
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| 关 键 词： | 复合PCR-变性高效液相色谱法(MPCR-DHPLC) 单核细胞增生李斯特菌 绵羊李斯特菌 英诺克李斯特菌 检测 |
MPCR-DHPLC Detection of Three Species of Pathogenic Listeria in Food |
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| WANG Yao,CAO Ji-juan,YAN Ping-ping,CAO Yuan-yin.MPCR-DHPLC Detection of Three Species of Pathogenic Listeria in Food[J].Food Science,2010,31(8):158. |
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| Authors: | WANG Yao CAO Ji-juan YAN Ping-ping CAO Yuan-yin |
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| Affiliation: | WANG Yao1,2,CAO Ji-juan2,YAN Ping-ping3,CAO Yuan-yin1(1.Institute of Plant Immunology,Shenyang Agricultural University,Shenyang 110161,China,2.Liaoning Entry-Exit Inspection , Quarantine Bureau,Dalian 116001,3.College of Life Science,Liaoning Normal University,Dalian 116029,China) |
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| Abstract: | A high-throughput and rapid multiplex PCR-denaturing high performance liquid chromatographic(MPCR-DHPLC) method was presented for the detection of Listeria monocytogenes,Listeria ivanovii and Listeria innocua in food.Respective primers specific to target genes of Listeria monocytogenes,Listeria ivanovii and Listeria innocua were designed and synthesized,followed by multiplex PCR amplification and DHPLC analysis.Along with repeatability experiments,specificity experiments were performed using 94 reference st... |
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| Keywords: | MPCR-DHPLC Listeria monocytogenes Listeria ivanovii Listeria innocua detection |
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