谱红细胞库的建立与应用

目的　通过对献血员和血站职工等群体普查ABO、Rh、MNSs、Duffy等 12个血型系统抗原 ,建立谱红细胞库和谱红细胞反应格局 ,用于临床鉴定特异性抗体。方法　选用盐水、菠萝酶和间接抗人球蛋白法。结果　筛出 9组O型Rh血型遣传式 (R1R1、R2 R2 、R1R2 、R1RZ、R2 RZ、R1r、R2 r、rr、rr′)和 2 9种抗原 (D、C、E、c、e、Cw、M、N、S、s、P1、Lea、Leb、Fya、Fyb、K、k、KPa、Kpb、Kpc、JSa、JSb、Dia、Xga、Lua、Lub、JKa、JKb、Jra)。结论　精选出 15个O型红细胞组成谱细胞格局 ,有 2 0种抗原鉴定抗体概率符合P <0 .0 5的要求。目的　通过对献血员和血站职工等群体普查ABO、Rh、MNSs、Duffy等 12个血型系统抗原 ,建立谱红细胞库和谱红细胞反应格局 ,用于临床鉴定特异性抗体。方法　选用盐水、菠萝酶和间接抗人球蛋白法。结果　筛出 9组O型Rh血型遣传式 (R1R1、R2 R2 、R1R2 、R1RZ、R2 RZ、R1r、R2 r、rr、rr′)和 2 9种抗原 (D、C、E、c、e、Cw、M、N、S、s、P1、Lea、Leb、Fya、Fyb、K、k、KPa、Kpb、Kpc、JSa、JSb、Dia、Xga、Lua、Lub、JKa、JKb、Jra)。结论　精选出 15个O型红细胞组成谱细胞格局 ,有 2 0种抗原鉴定抗体概率符合P <0 .0 5的要求。

Establishment and Application of Panel Red cell Bank

Objective To establish the bank and reaction pattern of panel red cell for the clinical identification of specific antibody.Methods Twelve blood group antigens,such as ABO,Rh,MNSs and Duffy ,were screened by saline,enzyme and indirect anti human globulin methods.Results A panel red cell bank consisting of 29 kinds of blood group antigens(D,C,E,c,e,C w,M,N,S,s,P 1,Le a,Le b,K,k,Kp a,Kp b,Kp c,JS a,JS b,Fy a,Fy b,Di a,Xg a,Lu a,Lu b,Jk a,Jk b,Jr a)and 9 groups of O type Rh genetypes(R 1R 1,R 2R 2,R 1R 2,R 1r,R 2r,R 1R z,R 2R z,R 2R z,rr,rr 1)was established.On the basis of this,a reaction pattern consisting of 15 kinds of group ed cells was also established.Conclusion The probability of identifying antibodies by the 20 kinds of antigens in the bank meet related requirment(P<0.05).