| 多重PCR法对乳制品中3种致病菌的同时快速检测 |
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| 引用本文： | 杨军,张驰,刘新梅.多重PCR法对乳制品中3种致病菌的同时快速检测[J].中国乳品工业,2010,38(4). |
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| 作者姓名： | 杨军 张驰 刘新梅 |
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| 作者单位： | 南京市产品质量监督检验院,国家农副产品监督检验中心,南京,210028 |
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| 摘 要： | 通过调整混合培养基的配比摸索乳制品中金黄色葡萄球菌、沙门氏菌和志贺氏菌的快速共增菌条件,并选择稳定高效的细菌基因组DNA提取方法,然后根据三种目标致病菌的保守基因分别设计多重PCR引物,对致病菌进行多重PCR检测.结果表明,方法具有良好的针对目标致病菌的特异性,检测限可达102mL1.且反应体系各组分间未见交叉干扰.对人工污染的乳品盲样检测验证了方法的实用性.
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| 关 键 词： | 沙门氏菌 金黄色葡萄球菌 志贺氏菌 多重PCR |
Multiplex PCR method for simultaneous detection of three bacteria in dairy products |
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| YANG Jun,ZHANG Chi,LIU Xin-mei.Multiplex PCR method for simultaneous detection of three bacteria in dairy products[J].China Dairy Industry,2010,38(4). |
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| Authors: | YANG Jun ZHANG Chi LIU Xin-mei |
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| Affiliation: | YANG Jun,ZHANG Chi,LIU Xin-mei(Nanjing Insititute of Supervision & Testing on Product Quality,Nanjing 210028,China) |
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| Abstract: | In the present study,we first optimized conditions for simultaneous enrichment of Staphylococcus aureus,Salmonella spp.and Shigella spp.in dairies,and then selected a stable and efficient method to extract genomic DNA.Subsequently,a multiplex PCR approach was settled,aiming at the conserved genes of target pathogens,respectively.This multiplex RTi-PCR procedure was verified to be specific to the target bacteria without cross interference,and the detection limit was 102 mL-1 for each pathogen.Finally,it was ... |
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| Keywords: | Salmonella Staphylococcus aureus Shigella multiplex PCR |
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