| 青蒿琥酯对人宫颈癌HeLa和Siha细胞辐照后DNA损伤水平的影响 |
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| 引用本文： | 周媛媛,封阳,张旭光,朱巍,倪倩影,耿冲,陈光烈,罗居东,樊赛军,曹建平.青蒿琥酯对人宫颈癌HeLa和Siha细胞辐照后DNA损伤水平的影响[J].辐射研究与辐射工艺学报,2011,29(1):33-36. |
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| 作者姓名： | 周媛媛 封阳 张旭光 朱巍 倪倩影 耿冲 陈光烈 罗居东 樊赛军 曹建平 |
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| 作者单位： | 周媛媛,封阳,朱巍,倪倩影,耿冲,陈光烈,罗居东,樊赛军,曹建平,ZHOU Yuanyuan,FENG Yang,ZHU Wei,NI Qianying,GENG Chong,CHEN Guanglie,LUO Judong,FAN Saijun,CAO Jianping(苏州大学放射医学与公共卫生学院放射医学与防护重点实验室,苏州,215123);张旭光,ZHANG Xuguang(徐州市肿瘤医院,徐州,221002) |
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| 基金项目： | 国家自然科学基金资助项目，长江学者和创新团队发展计划 |
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| 摘 要： | 为研究青蒿琥酯联合照射对人宫颈癌HeLa和Siha细胞DNA损伤的影响，取对数生长期细胞，通过MTT比色法测定不同浓度青蒿琥酯作用24h对HeLa和Siha细胞生长的抑制效应，将细胞分为单纯照射组和联合治疗组，每组分别给予0、2、4、6Gy四个剂量的60↑Coγ射线照射，应用单细胞凝胶电泳（SCGE）法检测青蒿琥酯对HeLa和Siha细胞照射后DNA损伤的影响。MTT结果表明，青蒿琥酯对宫颈癌HeLa和Siha细胞的生长抑制呈剂量依赖性。单细胞凝胶电泳法检测发现：联合治疗组HeLa细胞经0、2、4和60↑Coγ射线辐照后彗星细胞的尾部DNA百分数及Olive尾矩明显大于单纯照射组，差别有统计学意义（P〈0．05），而Siha细胞联合治疗组与单纯辐照组相比，无明显差异。实验结果表明，青蒿琥酯对p53突变型予宫颈癌HeLa细胞具有明显的放射增敏作用，而对p53野生型子宫颈癌Siha细胞没有明显的放射增敏作用。
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| 关 键 词： | 青蒿琥酯 放射增敏 DNA损伤 寓颈癌HeLa细胞 |
Study on the effect of artesunate combined with irradiation on DNA damage of HeLa and Siha cells of human cervical cancer |
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| ZHOU Yuanyuan,FENG Yang,ZHANG Xuguang,ZHU Wei,NI Qianying,GENG Chong,CHEN Guanglie,LUO Judong,FAN Saijun,CAO Jianping.Study on the effect of artesunate combined with irradiation on DNA damage of HeLa and Siha cells of human cervical cancer[J].Journal of Radiation Research and Radiation Processing,2011,29(1):33-36. |
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| Authors: | ZHOU Yuanyuan FENG Yang ZHANG Xuguang ZHU Wei NI Qianying GENG Chong CHEN Guanglie LUO Judong FAN Saijun CAO Jianping |
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| Affiliation: | ZHOU Yuanyuan1 FENG Yang1 ZHANG Xuguang2 ZHU Wei1 NI Qianying1 GENG Chong1 CHEN Guanglie1 LUO Judong1 FAN Saijun1 CAO Jianping1 1(College of Radiological Medicine and Public Health,Soochow University,Suzhou 215123,China) 2(The Tumor Hospital of Xuzhou,Xuzhou 221002,China) |
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| Abstract: | In order to investigate the effect of artesunate combined with irradiaton on DNA damage of HeLa and Siha cells of human cervical cancer,HeLa and Siha cells were cultured in vitro and exposed to different concentration of artesunate for 24 h and MTT assay was used to observe the inhibitory effect of different concentrations of artesu-nate on the proliferation of HeLa and Siha cells.The cells were divided into 2 groups as the irradiated group and the union treatment group.Here it was set up four absorbed dose... |
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| Keywords: | Artesunate Radiosensitivity DNA damage Cervical cancer HeLa cell |
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