正交试验法优化超声提取枣核总黄酮

目的：采用正交试验法，优选枣核中总黄酮的最佳超声提取工艺。方法：以芦丁为对照品，采用分光光度法进行测定，以提取液中黄酮含量作为考查指标，对影响黄酮提取工艺的因素进行了研究。首先，通过单因素试验研究了超声波功率、超声提取时间、料液比和提取溶剂乙醇体积分数对提取效果的影响，然后，利用正交试验法优化最佳提取工艺条件。结果：影响枣核黄酮提取的主要因素是乙醇体积分数与超声波功率，正交试验结果表明最佳提取工艺参数为：超声波作用功率是450W、超声波作用时间为30min、料液比1:50、乙醇体积分数50%。在此实验条件下，枣核黄酮粗品的提取率为7.04%，粗品中黄酮含量为1.59%。结论：采用正交试验-超声提取工艺，能有效提高枣核黄酮的提取效率，具有良好的应用前景。

Optimization by Orthogonal Array Design of Ultrasonic Extraction of Total Flavonoids from Chinese Date Seeds

Objective: The optimal conditions for ultrasonic extraction of total flavonoids from Chinese date seeds were investigated by orthogonal array design.Methods: The content of total flavonoids was determined spectrometrically using rutin as reference substance.The effects of ultrasonic power,extraction time,material/liquid ratio,ethanol concentration on the content of total flavonoids in extracts were investigated by one-factor-at-a-time method,and the extraction conditions were optimized by orthogonal array design.Results: Ethanol concentration and ultrasonic power were the key factors that affect the extraction of total flavonoids.The optimal extraction conditions were obtained as follows: ultrasonic power 450 W,treatment time 30 min,material/liquid ratio 1:50 and ethanol concentration 50%.Under these conditions,the extraction yield of crude total flavonoids from Chinese date seeds was 7.04% and the content of total flavonoids in crude extracts 1.59%.Conclusion: The optimized process remarkably increases the extraction yield of total flavonoids from Chinese date seeds and has a promising prospect for practical applications.