Purification and characterization of extracellular polysaccharide from activated sludges |
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| Authors: | N.J. Horan C.R. Eccles |
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| Affiliation: | 1. Key Laboratory of the Three Gorges Reservoir Region’s Eco-Environments of MOE, Chongqing University, Chongqing 400045, China;2. Key Laboratory of Reservoir Aquatic Environment of CAS, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China;1. School of Environmental Science and Engineering, Huazhong University of Science and Technology (HUST), Wuhan, 430074, PR China;2. Hubei Provincial Engineering Laboratory of Solid Waste Treatment, Disposal and Recycling, Wuhan, 430074, PR China;1. College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China;2. Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of TCM, PR China;3. Engineering & Technology Research Center for Chinese Materia Medica Quality of the Universities of Guangdong Province, PR China;1. Beijing Key Laboratory of Fuels Cleaning and Advanced Catalytic Emission Reduction Technology, School of Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing 102617, People’s Republic of China;2. Chinese Academy for Environmental Planning, Ministry of Environmental Protection, Beijing 100012, People’s Republic of China;3. Department of Environmental Engineering, Peking University, Beijing 100871, People’s Republic of China;4. The Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, People’s Republic of China |
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| Abstract: | A three stage purification process has been developed for soluble, activated-sludge exocellular polymers which results in a polysaccharide fraction substantially free of nucleic acids and protein, with the residual protein probably bound as a glycoprotein. Characterization and comparison of purified exopolysaccharide fractions from five different effluent treatment works revealed many similarities both in terms of monomer composition and molecular weight distribution. Only five monomers were detected, namely: glucose, galactose, mannose, glucuronic acid and galacturonic acid, and all the polysaccharide fractions were of high molecular weight, ranging from 3 × 105 to 2 × 106. |
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| Keywords: | polysaccharide flocculation purification extracellular polymer |
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