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Microbial community analyses using a simple, rapid detection method for DNA fingerprints with a fluorescence scanner |
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| Authors: | Ikeda Seishi Roberts David Mcl Watanabe Kazuo N Ytow Nozomi |
| Affiliation: | Gene Research Center, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8572, Japan. sikeda@gene.tsukuba.ac.jp |
| Abstract: | A simple detection procedure was developed for terminal restriction fragment length polymorphism and ribosomal intergenic spacer analysis for microbial community analysis. A fluorescence scanner enables generation of fingerprinting data in these methods within 3 h of obtaining PCR products of microbial communities. |
| Keywords: | fluorescence detection ribosomal intergenic spacer analysis (RISA) soil terminal restriction fragment length polymorphism (T-RFLP) |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |