Isovaleric acid as a precursor of odd numbered iso fatty acids in Tetrahymena |
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Authors: | Robert L Conner Kim-Eng Koo J R Landrey |
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Affiliation: | (1) Department of Biology, Bryn Mawr College, 19010 Bryn Mawr, Pennsylvania |
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Abstract: | Tris isovalerate-supplementedTetrahymena pyriformis W showed no qualitative change in fatty acid composition; however, an increase in polar lipids that contain odd numbered
iso acids (C13, C15, C17, C19) occurred. This change was accompanied by a decrease in the proportional amount of even numbered normal acids (C14, C16, C18). The neutral and polar lipids from cells incubated with 1-14C] isovaleric acid were found to contain radioactivity. The methyl esters of the saturated fatty acids obtained from the polar
lipids by alkaline methanolysis were separated by reversed phase chromatography, the identities confirmed by gas chromatography-mass
spectrometry, and the specific activities determined. Iso acids were found to be the most heavily labeled materials. In addition
to ceramide, two sphingolipid components were detected. One yielded saturated fatty acids after acidic methanolysis, while
the other contained >93% α-hydroxy fatty acids. Radioactivity was noted in the long chain base fraction derived from the sphingolipids.
Progressive growth inhibition occurred as the isovalerate concentration was increased in the culture medium; however, the
ciliates were morphologically indistinguishable from unsupplemented cells. |
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