Effect of protein depletion on the VLDL triacylglycerol secretion and apoprotein synthesis by the perfused liver from pregnant rats |
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Authors: | H Tournier G Yaryour O Mercuri M E De Tomás |
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Affiliation: | (1) Instituto de Investigaciones Bioquimicas de la Plata (INIBIOLP), Facultad de Ciencias Médicas, UNLP-CONICET, calles 60 y 120, 1900 La Plata, Argentina;(2) Present address: Carrera del Investigador Científico, Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina |
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Abstract: | The effect of protein depletion in the pregnant rat on the polyunsaturated fatty acid incorporation into very low density
lipoproteins (VLDL) has been investigated. The apoprotein pattern of these particles was determined. In in vivo experiments
the amounts of serum and liver triacylglycerol were determined. VLDL were isolated and their apo C concentration calculated.
In in vitro experiments the radioactivity of 3H] leucine incorporated into VLDL apoproteins was measured. The results show that protein depletion during pregnancy promotes
a drastic increase in serum and liver triacylglycerol. The VLDL isolated from these animals show an increase in the triacylglycerol/protein
ratio and a decrease in their content of apo C. Meanwhile, a significant reduction in the 3H]leucine incorporation into apo C peptides by the perfused liver of protein depleted rats was detected. On the other hand,
protein deprivation did not affect labeled linoleic and arachidonic acid incorporation into triacylglycerol of the newly secreted
VLDL. Taking these results together, let us deduce that a defective VLDL is secreted by the liver of the protein depleted
pregnant rats. The abnormal composition of these particles may influence its normal metabolism through their effects on lipoprotein
lipase and this fact could affect the normal supply of polyunsaturated fatty acids to the fetus. |
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