超高效液相色谱-四极杆飞行时间质谱筛查一起由生物碱引起的食物中毒

目的 采用超高效液相色谱-四极杆飞行时间质谱(UPLC-QTOF MS)技术对1起食物中毒事件的样本进行筛查，为患者救治及做好此类中毒的检测提供策略和依据。方法 患者的呕吐物、洗胃液和食物样本经酸化乙腈提取，C18固相萃取填料净化，利用UPLC-QTOF MS进行非依赖型全信息质谱数据采集，经UNIFI软件进行峰谱识别和谱库检索。结果 在5份人体样本中均检出莨菪碱，含量低于1 mg/kg,其中1人的胃液样本中筛出阿奇霉素；未发现莨菪碱代谢产物，与大鼠暴露实验结果一致。结论 由实验室检测结果推断本次事件是由莨菪碱引起的家庭食物中毒。UPLC-QTOF MS高分辨质谱筛查技术在处理由化学性物质引起的食物中毒突发事件中具有重要的实用价值。

Screening of a food poisoning caused by alkaloids by ultra-high-performance liquid chromatography quadrupole time of flight mass spectrometry

Objective Screening bio-samples from a food poisoning event based on ultra-high-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-QTOF MS), and to provide strategies and references for patient treatment as well as the event response. Methods Vomits, gastric lavage and food samples were extracted by acidic acetonitrile and purified by C18 sorbent and analyzed using UPLC-QTOF MS under data independent acquisition mode. Peak identification and spectrum library retrieval were carried out by the Unifi software. Results Hyoscyamine was detected in 5 human samples and the concentration was below 1 mg/kg. Azithromycin was also screened in one gastric lavage sample. No metabolites of hyoscyamine was found, which was consistent with the result of rat exposure experiment. Conclusion According to the screened result, it was inferred that this event was a family food poisoning caused by hyoscyamine. The UPLC-QTOF MS screening is of great significance in dealing with food poisoning emergencies.