Rapid multiplex single nucleotide polymorphism genotyping based on single base extension reactions and color-coded beads |
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| Authors: | Fujimura Naoko Kohara Yoshinobu Okano Kazunori Yohda Masafumi Kambara Hideki |
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| Affiliation: | Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo 184-0012, Japan. |
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| Abstract: | A single nucleotide polymorphism (SNP) typing method using color-coded beads is promising because it is easy to use and inexpensive. However, the present protocols are not suitable for clinical and diagnostic applications because they need centrifugation for bead-washing. Here, we developed a simplified protocol without a bead-washing procedure that enables SNP typing of PCR amplified fragments in only 30 min. |
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