| HPLC法快速测定墨西哥玉米和苏丹草中的糖类 |
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| 引用本文： | 谷淑波,樊广华,张晓艳.HPLC法快速测定墨西哥玉米和苏丹草中的糖类[J].现代仪器,2005,11(6):25-26,28. |
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| 作者姓名： | 谷淑波 樊广华 张晓艳 |
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| 作者单位： | 山东农业大学农学院,山东泰安,271018 |
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| 摘 要： | 本文采用高效液相色谱外标法,在Waters公司的Sugar-PakⅠ专用糖柱上,利用示差折光检测器,成功地同步测定出墨西哥玉米和苏丹草中糖类的含量。该方法快速准确,以水作流动相,柱温为90℃,相关系数在0.9998以上,加标回收率为98.7%~103.2%,精密度较高,RSD为2.67%~3.10%。测定结果表明,不同留茬和不同密度的牧草各糖组分的含量是不一样的。
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| 关 键 词： | 高效液相色谱法 墨西哥玉米和苏丹草 蔗糖 葡萄糖 果糖 甘露糖 |
Rapid determination carbohydrates in euchsaena mexicana schrad and sorghum sudanense by HPLC |
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| Gu Shubo,Fan Guanghua,Zhang Xiaoyan.Rapid determination carbohydrates in euchsaena mexicana schrad and sorghum sudanense by HPLC[J].Modern Instruments,2005,11(6):25-26,28. |
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| Authors: | Gu Shubo Fan Guanghua Zhang Xiaoyan |
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| Affiliation: | College of Agronomy, Shandong Agricultural University , Taian Shandong 271018 |
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| Abstract: | A rapid method for the determination of carbohydrates in Euchsaena mexicana Schrad and Sorghum sudanense by high-performance liquid chromatography is described.The chromatography was carried out on a Sugar-Pak Ⅰ(300mm×6.5mmi.d)(at 90℃),using deionized water(0.0001M Calcium EDTA) and refractive index detector.The correlation coefficients were above 0.9998.The recovery rate of determination were 98.7%～103.2% and the precision were 2.67%～3.10%.The result of determination showed the content of carbohydrates is diverse from different rotations. |
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| Keywords: | HPLC Euchsaena mexicana schrad and sorghum sudanense Sucrose Glucose Fructose Mannose 28 |
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