| 干法糖基化改性玉米醇溶蛋白产物的物化特性及在胶囊壳中的应用 |
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| 引用本文： | 张慧君,陈又铭,宫春宇,姜宁宁,沙迪昕,王文霞.干法糖基化改性玉米醇溶蛋白产物的物化特性及在胶囊壳中的应用[J].食品与机械,2018,34(10):104-108. |
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| 作者姓名： | 张慧君 陈又铭 宫春宇 姜宁宁 沙迪昕 王文霞 |
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| 作者单位： | 齐齐哈尔大学食品与生物工程学院，黑龙江 齐齐哈尔 161006；黑龙江省普通高校农产品加工重点实验室，黑龙江 齐齐哈尔 161006 |
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| 基金项目： | 黑龙江省自然科学基金面上项目（编号：C201455）；黑龙江省教育厅基本业务专项“粮头食尾”项目（编号：LTSW201701） |
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| 摘 要： | 利用菊粉对玉米醇溶蛋白进行干法糖基化改性,通过SDS-PAGE凝胶电泳、热分析(DSC)、傅里叶红外和扫描电子显微镜等手段考察改性前后蛋白的变化。结果表明:SDSPAGE电泳谱图可确认玉米醇溶蛋白与菊粉进行了有效的接合;傅里叶红外分析也确认糖基化改性后,玉米醇溶蛋白以共价键的形式接入了菊粉糖分子;DSC分析所得玉米醇溶蛋白膜及其改性产物膜的变性温度分别为188.47,194.02℃;扫描电子显微镜发现改性后膜的表面更加平整,无孔洞。参照《中华人民共和国药典(2015版)》中关于明胶胶囊壳的相关检测指标,对菊粉改性的玉米醇溶蛋白产物制成胶囊壳进行测定,该产品各项指标均达到要求。
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| 关 键 词： | 玉米醇溶蛋白 菊粉 糖基化改性 结构特性 胶囊壳 |
| 收稿时间： | 2018/3/21 0:00:00 |
Characterization of glycosylation of zein with inulin by dry-heating and its application in capsule shells |
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| ZHANGHuijun,CHENYouming,GONGChunyu,JIANGNingning,SHADixin,WANGWenxia.Characterization of glycosylation of zein with inulin by dry-heating and its application in capsule shells[J].Food and Machinery,2018,34(10):104-108. |
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| Authors: | ZHANGHuijun CHENYouming GONGChunyu JIANGNingning SHADixin WANGWenxia |
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| Affiliation: | College of Food Bioengineering, Qiqihar, Heilongjiang 161006, China; Key Laboratory of Processing Agricultural Products of Heilongjiang Province, Qiqihar University, Qiqihar, Heilongjiang 161006, China |
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| Abstract: | This study focuses on improvement mechanical properties of the zein films by glycosylation modifications under dry-heating conditions for capsule shells. The characterization of zein-inulin were analyzed by SDS-PAGE. DSC, FT-IR and SEM. The result indicates that the tensile strength of zein-inulin were 2.46 times of non-modifified zein. The formation of conjugates was verified by SDS-PAGE, infrared spectrum, and showed that the zein could joint the inulin by glycosylation with the covalent bound. The thermal denaturation temperature of zein was increased by DSC. It indicates that the thermal stability increases. Zein and zein-inulin films were scaned by SEM. The surface of zein-inulin films'' microporous structure disappeared, and the smooth of zein-inulin film was better than of that zein. The capsule shells can be produced from zein and zein-inulin. The inspection items for the capsule shells met the requirements of the Pharmacopoeia of the People''s Republic of China 2015 Edition. |
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| Keywords: | zein inulin glycosylation characterization capsule shells |
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