响应面法优化热回流提取樟树叶中木脂素工艺

利用响应面法优化樟树叶中木脂素的热回流提取工艺。通过单因素实验考察乙醇浓度、料液比、回流次数以及提取温度对总木脂素提取量的影响。在单因素试验基础上,采用Box-Behnken中心组合方法设计试验优化提取工艺。结果表明,最佳提取工艺条件为:乙醇浓度87%、料液比1:11 g/mL、回流3次、提取温度70℃,此条件下木脂素实际提取量为(43.39±4.91)mg/g,与理论预测值44.27 mg/g的偏差较小,即实际值与预测值之间的拟合度较好,说明响应面法对樟树叶中木脂素热回流提取条件进行优化的可行性。

Optimization of Hot Reflux Extraction Process of Lignans in Cinnamomum camphora Leaves by Response Surface Methodology

Response surface analysis was proposed to optimize the heat reflux extraction process of lignans fromthe leaves of Cinnamomum camphora. The effects of ethanol concentration,solid-liquid ratio,time,reflux times and temperature on lignans yield were investigated by single factor experiments. Based on the single factor level determination,the Box-Behnken center combination method was applied to determine the optimization of the extraction. Results showed that the optimum extraction condition were as follows:Ethanol concentration 87%,solid-liquid ratio 1:11 g/mL,reflux times 3,the temperature 70℃. Under the optimizatiom conditions,the experiment yield of lignans was(43.39±4.91) mg/g,which had less cleviation with the predicted value(44.27 mg/g). The results demonstrated that it was practicable to optimize the heat reflux extraction process using response surface methodology.