白及中四类糖的定量检测

白及中糖类的含量对评价白及质量有重要意义,为此开展了白及中四类糖的定量检测研究。白及粉用适量水均匀润湿,加无水乙醇调至乙醇浓度为80% vol,超声提取,离心分离。清液除去乙醇后,用纯水定容,按GB5009.7检测还原糖和酸水解后检测非还原低聚糖含量。固体用淀粉酶水解后定容、过滤,清液加4倍体积的无水乙醇混匀后离心,其离心清液按GB 5009.9检测淀粉含量,固体按SN/T 4260检测粗多糖含量。结果表明,还原糖、非还原低聚糖、淀粉、粗多糖的RSD为1.12%、0.67%、3.66%、1.73%,回收率为103.41(98.14)%、93.76%、101.21%、96.16%,白及样品中还原糖、非还原低聚糖、淀粉、粗多糖含量为1.37%、4.46%、4.70%、42.50%。该方法符合相关糖类检测标准的精密度和准确度要求,可用于白及四类糖的定量检测。

Quantitative Detection of Four Sugars from Bletilla striata

The content of sugar in Bletilla striata was very important to evaluate its quality. Therefore,quantitative detection methods of 4 sugars were studied in this paper. Bletilla striata powder was mixed with distilled water uniformly,absolute alcohol was added to mixture and the volume fraction of alcohol was 80%. Mixture was extracted by ultrasound and centrifuged. After removal of alcohol from upper,the liquid was diluted to required volume with distilled water.The content of reducing sugar and non-reducing oligosaccharide after acid hydrolysis were quantitative detected by GB5009.7. After amylase hydrolysis of solids,diluted to required volume with distilled water and centrifuged. 4 times volume of absolute alcohol was added to upper clear liquid,mixed uniformly and centrifuged. Starch was detected in centrifuged clear liquid by GB5009.9. Rude polysaccharide was detected in solid by SN/T 4260. Results showed that,the RSD(relative standard deviation)experiment result of reducing sugar,non-reducing oligosaccharide,starch and rude polysaccharide were 1.12%,0.67%,3.66%,1.73%,recovery rate were 103.41(98.14)%,93.76%,101.21%,96.16%. The content of reducing sugar,non-reducing oligosaccharide,starch and rude polysaccharide was 1.37%,4.46%,4.70%,42.50% in Bletilla striata sample. The accuracy and precision of this method conformed to related national quantitative detection standards of carbohydrate and could be used for quantitative detected 4 carbohydrates in Bletilla striata.