桑葚花色苷超高压提取工艺优化及其组分分析

以桑葚为原料采用超高压法提取花色苷,通过响应面优化提取工艺,并用高效液相色谱法分析桑葚提取液中花色苷种类和含量。结果表明:超高压提取桑葚花色苷的最佳工艺条件为:压力270 MPa、保压时间6 min、料液比1:35 g/mL、乙醇浓度62%,在此条件下桑葚花色苷得率为(4.93±0.12)mg/g,较热回流提取和超声波提取得率分别提高了25.93%和18.26%。从桑葚提取液中鉴定出4种花色苷单体分别为飞燕草色素、矢车菊色素、牵牛花色素、芍药色素,其含量分别为1.32、2.05、0.27、0.25 mg/g。高效液相色谱分析花色苷组分为桑葚花色苷进一步开发利用提供理论依据。

Optimization of Anthocyanins Extraction by Ultra-high Pressure from Mulberry and Its Compositions Analysis

The fruits of the mulberry were extracted by ultra-high pressure technique to obtain anthocyanins. The extraction process was optimized by response surface methodology. High performance liquid chromatography(HPLC)was applied to analyze the types and contents of anthocyanidins. The results showed that the optimum extraction parameters to achieve the highest yield of anthocyanins of(4.93±0.12) mg/g from mulberry powder was obtained under an extraction pressure of 270 MPa,pressure-holding time of 6 min,solid-to-liquid ratio of 1:35 g/mL,and ethanol concentration of 62%,which were 25.93% and 18.26% higher than those obtained with hot reflux extraction method and ultrasonic extraction method,respectively. The anthocyanins in mulberry were identified 4 types anthocyanins monomers including delphinidin,cyanidin,petunidin and peonidin. Their contents were 1.32,2.05,0.27,0.25 mg/g,respectively. The HPLC analysis of anthocyanins components would provid a theoretical basis for further development and utilization of pigment from mulberry.