超声波辅助法提取假苹婆树叶总黄酮及其清除羟自由基能力

利用超声波辅助提取技术研究假苹婆树叶总黄酮的提取工艺及其抗氧化活性。以总黄酮得率为研究对象,考察了料液比,乙醇体积分数、提取功率、提取时间及提取温度对提取效果的影响,通过正交试验对提取工艺进行了优化。结果表明,最佳提取工艺条件为:乙醇体积分数为60%,提取功率为60 W,料液比为1:25(g/mL),70℃提取65 min,该条件下总黄酮得率为2.36%±0.0002%。抗氧化试验表明,假苹婆提取液对羟自由基的清除能力随着提取液中总黄酮浓度的增大而增强,当假苹婆树叶总黄酮的质量浓度为4.92 mg/mL时,清除率达到73.58%。

Ultrasonic-assisted Extraction of Total Flavonoids from Sterculia Lanceolata and Scavenging Capacity on Hydroxyl Free Radical

The ultrasonic-assisted extraction of total flavonoids from Sterculia lanceolata and antioxidant activity were studied. The influences of the ratio of Sterculia lanceolata to solvent,ethanol concentration,extraction power,extraction time and extraction temperature were investigated by single factor experiment,and the extraction process were optimized by orthogonal test with total flavonoids yield as index. The results showed that the optimal of extraction were as follows:the ratio of sterculia lanceolata to solvent was 1:25 (g/mL),ethanol concentration was 60%,extraction power was 60 W,the extraction was token place at 70℃ for 65 min. Under these conditions,the yield of total flavonoids was 2.36%±0.0002%. Antioxidant test showed that the hydroxyl radical scavenging activity increased with the increasing concentration of total flavonoids from the leaves of sterculia lanceolata. At the concentration of 4.92 mg/mL,the total flavonoids exhibited a maximum hydroxyl radical scavenging rate of 73.58%.