蓝莓果渣花色苷提取工艺优化及抗氧化活性比较研究

以蓝莓干果渣为原料,酸化乙醇为溶剂,应用超声辅助法提取花色苷,利用pH示差法测定花色苷含量,通过单因素实验和响应面试验优化得出蓝莓干果渣花色苷提取工艺条件,比较相同干重的蓝莓干果渣、湿果渣采用超声辅助提取和无超声辅助工艺得到的提取液的总多酚、花色苷含量及抗氧化活性。结果表明,蓝莓干果渣最佳提取工艺为液料比(31:1)mL/g,乙醇浓度63%,pH2.6,超声功率500 W,提取时间20 min,在此条件下蓝莓果渣提取液中花色苷含量为498.84 mg/100 g。相同原料,超声辅助提取工艺提取液较无超声辅助提取工艺提取液的总多酚和花色苷含量多;相同工艺条件下,相同干重的湿果渣总多酚和花色苷含量较干果渣低,但却拥有更高的抗氧化活性。

Optimization of Extraction Conditions of Anthocyanin from Blueberry Pomace and Its Antioxidant Activity

Using blueberry dried pomace as raw material,acidified ethanol as solvent,extracting anthocyanin by ultrasonic assisted method,and determining its content by pH differential method,and the optimizing extraction conditions of anthocyanin from blueberry dried pomace were determined by single factor test and Box-Behnken design. And the antioxidant activity of blueberry dried pomace and wet pomace with the same dry weight were compared by ultrasound-assisted extraction and no ultrasonic assisted extraction. The results showed that,the optimized extraction process of blueberry dried pomace were liquid-solid ratio of 31 mL/g,aethyl-alcohol concentration of 63%,pH of 2.6,ultrasonic power of 500 W,and sonication time of 20 min.Under this condition,the anthocyanin content in the blueberry pomace extract was 498.84 mg/100 g. With the same raw materials,the contests of total polyphenols and anthocyanins in the extract by ulttasonic assistred was more than the process without ultrasonic assisted. With the same process conditions,the contents of polyphenols and anthocyanins of wet pomace on the same dry weight were lower than that of dried pomace,but had a higher antioxidant activity.