托盘根不同溶剂萃取物的活性成分及其体外抗氧化活性

采用溶剂萃取法对托盘根水提物中的活性物质进行萃取,并测定不同溶剂萃取物中总黄酮、总皂苷和总酚酸的含量,运用DPPH、ABTS和超氧阴离子等体外抗氧化活性模型,研究托盘根不同溶剂萃取物的体外抗氧化活性。结果显示,托盘根正丁醇萃取物中总黄酮、总皂苷、总酚酸含量分别为5.06%、7.41%、6.18%,高于氯仿萃取物和乙酸乙酯萃取物。体外抗氧化活性实验表明,乙酸乙酯萃取物清除DPPH和超氧阴离子的作用最强,IC₅₀分别为94.64 μg/mL和50 μg/mL。正丁醇萃取物清除ABTS的作用最强,IC₅₀值为66.43 μg/mL。结论:托盘根提取物中含有黄酮类、皂苷类、酚酸类成分,氯仿萃取物、乙酸乙酯萃取物和正丁醇萃取物具有较好的体外抗氧化活性,为进一步开发托盘根为天然抗氧化剂或健康食品提供理论基础。

Active Composition of Different Solvent Extracts of Rubus crategefolius Bunge. Root and Their Antioxidant Activity in Vitro

The bioactive constituents of Rubus crategefolius Bunge. were extracted with solvent extraction method. The contents of total flavonoids,total saponins and total phenolic acid were also measured. The in vitro antioxidant activity of each extract was determined by DPPH,ABTS and superoxide anion scavenging assays. The results showed that the contents of total flavonoids,total saponins and total phenolic acid of n-buthanol extract were 5.06%,7.41% and 6.18% and it was higher than those of chloroform extract and ethyl-acetate extract. Among the extracts,ethyl-acetate extract showed higher DPPH and superoxide anion scavenging activities and its IC₅₀ were 94.64 μg/mL and 50 μg/mL,respectively. n-Buthanol extract showed higher ABTS scavenging activity than other extracts and IC₅₀ was 66.43 μg/mL. In conclusion,the roots of R. crataegifolius Bunge. contains flavonoids,saponins and phenolic acid. Furthermore,chloroform extract,ethyl-acetate extract and n-buthanol extract of roots of R. crataegifolius Bunge. had shown strong antioxidant activity. The data could provide the theory basis for the developing R. crataegifolius Bunge. as natural antioxidant or functional food.