金槐芦丁聚酰胺树脂纯化工艺优化

为探索聚酰胺树脂纯化芦丁的工艺条件,以金槐槐米为原料,考察上样液浓度、上样液流速、洗脱剂浓度、洗脱剂体积4个因素对芦丁精制的影响,确定最佳的分离纯化工艺。结果表明:在上样液浓度0.286 mg/mL,上样液流速1.0mL/min,洗脱剂80%乙醇,洗脱体积2.5BV条件下,芦丁得率为55.49%,纯度为99.08%。

Purification of Rutin from Flos Sophora

In order to comprehensively utilize Flos Sophora resources and industrialized large-scale production, the development of optimal conditions for the purification of rutin by polyamide resins is highly desirable. Paraphlomis jauanica was used as the raw material, and four factors including concentration of sample solution, flow rate of the sample solution, ethanol concentration of the eluent and elution volume were investigated to ascertain its influence on the purification of rutin. When the concentration of the sample was 0.286 mg/mL, flow rate of the sample solution was 1.0 mL/min, with eluent of 80% ethanol and elution volume of 2.5 BV. Finally, the yield of rutin was 55.49% and the purity was 99.08%.