卵黄高磷蛋白对壳聚糖膜基质上MC3T3-E1细胞增殖的影响

本研究制备了壳聚糖和氧化石墨烯/壳聚糖复合膜,并采用CCK-8法、Annexin V/PI双染法和PI染色检测了蛋黄中提取的卵黄高磷蛋白对MC3T3-E1细胞在该复合膜基质上的生理活动的影响。结果表明:壳聚糖膜对细胞无毒性影响,并通过提高S期细胞所占比例,使MC3T3-E1细胞活性提高了31.92%,抑制细胞凋亡,使凋亡率下降45.78%;氧化石墨烯/壳聚糖膜会提高细胞的凋亡率,氧化石墨烯含量越高,促进凋亡越明显,凋亡率在氧化石墨烯含量为2%时最高,为17.64%;在壳聚糖膜基质上,100 μg/mL卵黄高磷蛋白对MC3T3-E1细胞促进增殖作用最显著(p<0.05),细胞活性提高81.59%,同时降低了细胞的凋亡比例;蛋白浓度为500 μg/mL时,细胞活性下降4.47%,促进了细胞凋亡。综上所述,壳聚糖和低浓度卵黄高磷蛋白均能提高MC3T3-E1细胞的活性。

Effect of Phosvitin on the Proliferation of MC3T3-E1 Cells on the Chitosan Membrane

In this study,the graphene oxide/chitosan composite film was prepared,and the effects of the phosvitin extracted from the yolk on the physiological activity of MC3T3-E1 cells on the matrix of the composite film were detected by CCK-8,Annexin V/PI double staining and PI staining. The results showed that,the chitosan film had no toxic effect on the cells,and increased the activity of MC3T3-E1 cells by 31.92% by increasing the proportion of S cells,inhibited apoptosis and reduced the apoptosis rate by 45.78%. The graphene oxide/chitosan films could increase the apoptosis rate of cells. The higher the content of oxidation stone was,the more obvious the apoptosis could be promoted. When the graphen oxide content was 2%,the apoptotic rate was the highest,which was 17.64%.On the matrix of chitosan film,100 μg/mL phosvitin had the most significant effect on the proliferation of MC3T3-E1 cells and the cell activity increased by 81.59%,and the percentage of cell apoptosis had decreased. The cell activity decreased and cell apoptosis promoted by 4.47% when the concentration of protein was 500 μg/mL. In summary,chitosan and low concentration of phosvitin could promote the proliferation of MC3T3-E1 cells.