鲎血细胞蛋白营养学评价及其水解肽体外活性

对鲎血细胞蛋白(TTBCP)进行营养学评价,并测定TTBCP水解物的体外抗氧化性、α-葡萄糖苷酶和乙酰胆碱酯酶的抑制活性。结果显示:TTBCP中必需氨基酸优于成人的FAO/WHO推荐模式,基本符合小孩的FAO/WHO推荐模式,其中必需氨基酸与总氨基酸的比值(E/T)为31.2%,氨基酸评分(AAS)为68.1,蛋白质效率比(PER)小于2.0。TTBCP水解肽对α-葡萄糖苷酶的半数抑制浓度(IC₅₀)为0.199 mg/mL,抑制率最高达到83.8%,对乙酰胆碱酯酶同样有抑制作用;TTBCP水解肽能清除超氧阴离子自由基、DPPH自由基和羟自由基,当浓度小于0.75 mg/mL的范围时,还原能力大于维生素C。由此可见,TTBCP具有复合功效,可作为糖尿病的保健食品和药品开发利用。

Nutritional Evaluation of Blood-cell Protein from Tachypleus tridentatus and Activities of Its Hydrolysate in Vitro

The nutritional evaluation of blood-cell protein from Tachypleus tridentatus(TTBCP)was carried out,and its antioxidant activities,the inhibitory activities on α-glucosidase and acetylcholinesterase were measured in vitro. The results showed that the essential amino acids in TTBCP were superior to the adult's FAO/WHO-recommending model,basically conforming to the child's FAO/WHO-recommendating model. The ratio of essential amino acids to total amino acids(E/T)was 31.2%,the amino acid score(AAS)was 68.1,the protein efficiency ratio(PER)was less than 2.0. The half inhibitory concentration(IC₅₀)of TTBCP on α-glucosidase was 0.199 mg/mL with the highest inhibition rate of 83.8 mg/mL. TTBCP also had inhibitory effect on cholinesterase. The hydrolysate peptide of TTBCP could scavenge the superoxide anion radicals,DPPH radicals and hydroxyl radicals,its reduction ability was greater than vitamin C when the concentration was less than 0.75 mg/mL. So,TTBCP could be developed into a health food and medicine for diabetes mellitus owing to its comprehensive efficacy.