响应面法优化酸枣仁多糖的脱色工艺

利用响应面法优化酸枣仁多糖的脱色工艺。首先通过静态吸附法选择具有较好脱色工艺的树脂类型,然后通过单因素实验结果,将上样质量浓度(mg/mL)、上样量(mL)及洗脱流速(BV/h)作为工艺优化中的主要影响因素,并以多糖脱色率和多糖保留率综合得出的归一值(OD)作为响应值,利用Box-Behnken试验设计,响应面分析优化酸枣仁多糖脱色工艺。结果表明:选用D101大孔吸附树脂,当上样质量浓度为2 mg/mL,上样量为3.5 mL,洗脱流速为4 BV/h时脱色效果最佳,此条件下多糖脱色率和多糖保留率分别为61.32%和87.05%,二者综合得出的归一值OD值为0.56(模型预测值0.55),说明该工艺可行性良好。

Optimization of Decoloration Process of Zizyphi Spinosae Semen Polysaccharide by Response Surface Methodology

Zizyphi Spinosae Semen polysaccharide decoloration process was optimized by the method of response surface optimization. Firstly,the resin type with a well decolorization process was selected by static adsorption method. Then the sample concentration(mg/mL),sample volume(mL)and elution velocity(BV/h)were used as the main factors in the process optimization by single factor experiment. Based on response value(OD)of polysaccharide decolorization rate and retention rate,response surface method(RSM)was used to analyze optimization the decolorization process by Box-Behnken design(BBD). The results showed that the optimized decolorization parameters with D101 macroporous adsorption resin were 2 mg/mL for the sample concentration,3.5 mL for the volume,and 4 BV/h for the elution flow rate. Under the condition,the value of polysaccharide decolorization rate and retention rate were 61.32% and 87.05%,respectively,and the OD value was 0.56(the predicted value of 0.55),which indicated the optimized decoloration method was feasible.