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高剪切分散乳化技术对大豆分离蛋白凝胶中水分状态的影响
引用本文:刘俊梅,王丹,李琢伟,于立梅,王庆,张岚,杨盼盼,王辉,王璐,于寒松,胡耀辉.高剪切分散乳化技术对大豆分离蛋白凝胶中水分状态的影响[J].现代食品科技,2014,30(8):131-137.
作者姓名:刘俊梅  王丹  李琢伟  于立梅  王庆  张岚  杨盼盼  王辉  王璐  于寒松  胡耀辉
作者单位:吉林农业大学食品科学与工程学院,吉林长春 130118;吉林农业大学食品科学与工程学院,吉林长春 130118;长春职业技术学院,吉林长春 130033;仲恺农业工程学院轻工与食品学院,广东广州 510225;吉林农业大学食品科学与工程学院,吉林长春 130118;吉林医药学院公共卫生系,吉林吉林 132113;吉林农业大学食品科学与工程学院,吉林长春 130118;吉林农业大学食品科学与工程学院,吉林长春 130118;吉林农业大学食品科学与工程学院,吉林长春 130118;吉林农业大学食品科学与工程学院,吉林长春 130118;吉林农业大学食品科学与工程学院,吉林长春 130118
基金项目:现代农业产业技术体系建设专项资金资助(s240-4)
摘    要:为了探讨高剪切分散乳化技术(High shear dispersing emulsification technology,HSDE)对TG酶诱导的大豆分离蛋白(Soy protein isolate,SPI)凝胶中水分状态的影响,本文采用低场核磁共振技术从水分分布、水分迁移、持水能力三个方面研究高剪切分散乳化技术对大豆分离蛋白凝胶体系中水分状态影响机制。结果表明:HSDE技术处理后制成的凝胶,其结合水的流动性增加了,但是自由水的流动性是降低的,剪切后凝胶中的自由水和结合水会发生向不易流动水迁移的现象。从成像图中可以看出剪切处理能使凝胶中水分分布均匀。从相关性角度分析,剪切与T21、T22、T23表现为极显著负相关,与凝胶的持水性呈正相关。在持水性探索过程中发现,HSDE技术能够提高TG酶诱导的大豆蛋白凝胶持水能力,在贮存过程中,HSDE技术能显著提高凝胶持水性。

关 键 词:低场核磁共振技术  高剪切分散乳化技术  水分状态  持水性  大豆蛋白凝胶
收稿时间:2014/3/27 0:00:00

Effect of High Shear Dispersing Emulsification Technology on the Water Status in Soy Protein Isolate Gel
LIU Jun-mei,WANG Dan,LI Zhuo-wei,YU Li-mei,WANG Qing,ZHANG Lan,YANG Pan-pan,WANG Hui,WANG Lu,YU Han-song and HU Yao-hui.Effect of High Shear Dispersing Emulsification Technology on the Water Status in Soy Protein Isolate Gel[J].Modern Food Science & Technology,2014,30(8):131-137.
Authors:LIU Jun-mei  WANG Dan  LI Zhuo-wei  YU Li-mei  WANG Qing  ZHANG Lan  YANG Pan-pan  WANG Hui  WANG Lu  YU Han-song and HU Yao-hui
Affiliation:College of Food Science and Engineering, Jilin Agriculture University, Changchun 130118, China;College of Food Science and Engineering, Jilin Agriculture University, Changchun 130118, China;Changchun Vocational Institute of Technology, Changchun 130033, China;College of Light Industry and Food Science, Zhongkai University of Agricultural and Engineering, Guangzhou 510225, China;College of Food Science and Engineering, Jilin Agriculture University, Changchun 130118, China;Department of Public and Health, Jilin Medical College, Jilin, 132113, China;College of Food Science and Engineering, Jilin Agriculture University, Changchun 130118, China;College of Food Science and Engineering, Jilin Agriculture University, Changchun 130118, China;College of Food Science and Engineering, Jilin Agriculture University, Changchun 130118, China;College of Food Science and Engineering, Jilin Agriculture University, Changchun 130118, China;College of Food Science and Engineering, Jilin Agriculture University, Changchun 130118, China
Abstract:In this study, the effects of high shear dispersing emulsification (HSDE) technology on the water status of transglutaminase-induced soy protein isolate (SPI) gel were investigated. Low-field nuclear magnetic resonance (LF-NMR) technology was used to study the effect of HSDE technology on the water status in SPI gels, in terms of water distribution, water mobility, and water holding capability (WHC). The results indicated that the mobility of bound water increased whereas that of free water decreased in the HSDE-treated gel. Additionally, bound and free water in HSDE-treated samples could be immobilized. Magnetic resonance imaging (MRI) revealed that water was distributed evenly in the gel treated with HSDE. According to the correlation analysis, HSDE showed a significant negative correlation with T21, T22, and T23, but it positively correlated with the WHC of the gel. Analysis of WHC showed that HSDE technology could improve the WHC of transglutaminase-induced SPI gel, which improved significantly during storage.
Keywords:low field-nuclear magnetic resonance  high shear dispersing emulsification technology  water Status  water holding capability  soy protein isolate gel
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