广州市售散装熟肉制品中细菌的耐药性及耐药基因研究

本文以广州市售散装熟肉制品为研究对象,通过16S rDNA序列分析及生理生化试验对菌株进行鉴定,同时检测菌株对四环素和复方新诺明的耐药性及耐药基因的分布。40份熟肉制品中共筛选出169株细菌,其中肠杆菌属、假单胞菌属和柠檬酸杆菌属所占比例较大。所有分离株中有55株(32.54%)对四环素表现出耐药,40株(23.67%)对复方新诺明表现出耐药。在7种四环素耐药基因中,tetM(14.20%)的检出率为最高,其次分别为tetA(10.06%)、tetS(5.92%)和tetB(2.96%);磺胺类耐药基因中sulI的检出率最高,为10.65%。169株受试菌中有19株(11.24%)同时含有两种或两种以上耐药基因。熟肉制品中耐药菌株及相关耐药基因的广泛分布表明这些食源性细菌已成为潜在的耐药基因库,并且可能通过食品链将耐药性传递给人类,从而引起人类的感染以及耐药菌株的流行。

The Antimicrobial Resistance and Resistant Genes in Bacteria from Bulk Cooked Meats in Guangzhou

The aerobic bacteria were isolated from cooked meats in Guangzhou, identified by 16S rDNA amplification and biochemical identification, and the antimicrobial resistance to tetracycline and trimethoprim/sulfamethoxazole and the prevalence of corresponding resistant genes were investigated in this study. Of the 169 isolates collected from 40 samples, Enterobacter, Pseudomons and Citrobacter were found more frequently in these cooked meats. Fifty-five (32.54%) of all isolates were resistance to tetracycline and 40 (23.67%) of them were resistance to trimethoprim/sulfamethoxazole. Among seven tetracycline resistance genes in this study, tetM (14.20%) was found in the highest frequency, followed by tetA (10.06%), tetS (5.92%)and tetB (2.96%), while sulI (10.65%) was observed in the highest frequency in trimethoprim/sulfamethoxazole resistance genes. Multiple antimicrobial resistance genes were detected in 19 of 169 (11.24%) isolates. The prevalence of resistance genes in isolates from cooked meats indicates that these bacteria might act as a reservoir of antimicrobial resistance genes and play a role in the dissemination of resistance along the food chain.