高效液相色谱法测定化妆品中间苯二酚和水杨酸

采用高效液相色谱法同时测定化妆品中间苯二酚和水杨酸。色谱条件:Kromasil C8(250 mm×4.6 mm i.d.,5μm)色谱柱,以流动相甲醇+0.01 mol/L磷酸氢铵缓冲溶液进行梯度洗脱,流速1.0 mL/min,检测波长275 nm。结果表明,在此条件下间苯二酚和水杨酸均在0.5～150μg/mL内与相应的峰面积具有良好的线性关系(相关系数r分别为0.999 7和0.999 8),线性回归方程分别为A=12 420ρ-6 301和A=15 500ρ-8 810,回收率在95.9%～103.5%,RSD为0.61%～0.88%。

Determination of resorcinol and salicylic acid content in cosmetics by reversed-phase high performance liquid chromatography

A reversed-phase high performance liquid chromatography method for simultaneous determination of resorcinol and salicylic acid in cosmetics was established.The sample was analyzed on a Kromasil C8 column(250 mm×4.6 mm i.d.,5 μm) with a detection UV wavelength of 275 nm.Gradient elution was carried out with mobile phase composed of methanol buffered with 0.01 mol/L ammonium dihydro-phosphate at a flow rate of 1.0 mL/min.Results showed that as the concentration of resorcinol and salicylic acid are in the same range of 0.5~150 μg/mL,the relationship between concentration and the corresponding peak area shows good linearity(r=0.999 7 and 0.999 8 respectively).The regression equations can be expressed as A=12 420 ρ-6 301 and A=15 500 ρ-8 810 respectively;and the recoveries of resorcinol and salicylic acid achieve 95.9%~103.5%.The RSD of the method is 0.61%~0.88%.