Determination of hydroperoxides and structures by high-performance liquid chromatography with post-column detection with diphenyl-1-pyrenylphosphine |
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| Authors: | Toshiaki Ohshima Anu Hopia J Bruce German Edwin N Frankel |
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| Affiliation: | (1) Department of Applied Chemistry and Microbiology, University of Helsinki, FIN-00014, Finland;(2) Department of Food Science and Technology, University of California, 95616 Davis, California;(3) Department of Food Science and Technology, Tokyo University of Fisheries, Konan 4, Minato-ku, 108 Tokyo, Japan |
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| Abstract: | A high-performance liquid chromatographic method, using post-column detection with diphenyl-1-pyrenyl-phosphine (DPPP), was
developed for the quantitative and qualitative determination of isomeric lipid hydroperoxides (OOH). The OOH eluted from a
normal-phase column were passed through a photodiode array detector and then mixed with DPPP solution in a reaction coil heated
at 80°C. DPPP oxide formed by the reaction with OOH was determined by monitoring the fluorescence intensity at 380 nm and
excitation at 352 nm. The conjugated diene OOH (13-cis, trans- and 9-cis, trans-OOH) and nonconjugated OOH (12-cis-trans- and 10-cis, trans-OOH) from photosensitized oxidation of methyl linoleate were determined in a molar ratio of 31∶29∶19∶21, respectively. However,
only the two conjugated hydroperoxides were detected by ultraviolet absorption at 234 nm. Further applications were carried
out for the determination of OOH of methyl oleate and methyl linolenate. This method proved to be useful for the determination
of the OOH containing both conjugated and nonconjugated diene structures. |
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