高糖胁迫下蜂蜜接合酵母碳代谢流的研究

研究蜂蜜接合酵母LGL-1在高糖胁迫下的代谢特性及代谢流变化。采用高效液相色谱等方法测定蜂蜜接合酵母LGL-1在葡萄糖质量浓度100 g/L和400 g/L条件下积累的代谢产物(酒精、海藻糖、甘油、苹果酸、α-酮戊二酸、柠檬酸及乳酸),通过构建代谢矩阵方程组,采用Matlab软件和归一法分析关键节点的代谢流分布情况。结果表明,蜂蜜接合酵母LGL-1高糖胁迫时的碳流量偏向流入海藻糖、甘油、乙醇和TCA循环的代谢支路,与常规培养相比,海藻糖、甘油和TCA循环的通量分别增加了50.00%,17.73%和4.79%,高糖培养与常规培养的乙醇代谢通量接近。在高糖胁迫条件下蜂蜜接合酵母LGL-1积累的海藻糖、甘油以及TCA循环的通量变化也反映海藻糖、甘油以及TCA循环对酵母细胞的重要保护功能。

Research on Carbon Metabolic Flow of Zygosacharomyces mellis under High Glucose Stress

Metabolic characteristics and metabolic flow of Zygosacharomyces mellis LGL-1 under high-glucose stress were studied.Metabolites accumulated under 100 g/L and 400 g/L glucose concentration,such as alcohol,trehalose,glycerin,malic acid,α-ketoglutarate,citric acid,and lactic acid were determined.Metabolic flow distribution of key nods were analyzed through construction of metabolic matrix equations,MATLAB software and normalization method.The results showed that the carbon flow of Z.mellis LGL-1 was towards the metabolism branch of trehalose,glycerol,alcohol synthesis and TCA cycle.Compared with the normal cultivation,the metabolic flux of trehalose,glycerol and TCA under high-glucose stress was increased by 50.00%,17.73%and 4.79%,respectively.But the metabolic flux of ethanol showed little difference.Trehalose and glycerin accumulation,flux change of TCA cycle under high-glucose stress reflected the important protective function to Z.mellis LGL-1.