| 不同发酵条件对重组猕猴桃果胶甲酯酶抑制剂表达量的影响 |
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| 引用本文： | 梅晓宏,高红岩,罗云波.不同发酵条件对重组猕猴桃果胶甲酯酶抑制剂表达量的影响[J].食品科技,2007,32(5):37-40. |
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| 作者姓名： | 梅晓宏 高红岩 罗云波 |
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| 作者单位： | 中国农业大学食品科学与营养工程学院,北京,100083 |
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| 摘 要： | 将已在毕赤酵母中成功表达的重组猕猴桃果胶甲酯酶抑制剂为研究对象,通过改变发酵培养时间、培养基装填量及培养基配方,对该重组蛋白的表达量进行研究。最终确定使用BMMY培养基、表达时间为96h、培养基装填量为10%时为最佳表达条件,在最佳表达条件下,该重组蛋白的表达量最高可达0.2g/L。
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| 关 键 词： | 果胶甲酯酶 抑制剂 发酵条件 蛋白表达 |
| 文章编号： | 1005-9989(2007)05-0037-03 |
| 修稿时间： | 2006年10月20 |
Effect on expression yield of the recombinant kiwi pectin methylesterase inhibitor in different fermentation conditions |
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| MEI Xiao-hong,GAO Hong-yan,LUO Yun-bo.Effect on expression yield of the recombinant kiwi pectin methylesterase inhibitor in different fermentation conditions[J].Food Science and Technology,2007,32(5):37-40. |
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| Authors: | MEI Xiao-hong GAO Hong-yan LUO Yun-bo |
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| Abstract: | In this paper,the expression yield of recombinant kiwi pectin methylesterase inhibitor which had been successfully expressed in Pichia pastoris was studied in different fermentation time,different loading volume and different medium.The optimal conditions were finally established as follows: BMMY medium,96 hours and 10% loading volume.The highest yield of this recombinant protein reached 0.2g/L in the above conditions. |
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| Keywords: | pectin methylesterase inhibitor fermentation condition protein expression |
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