中国狂犬病毒疫苗株CTN-1-V三个代次的GP基因序列分析

目的分析三个代次CTN-1-V病毒株糖蛋白(GP)基因部分序列,并与代表性街毒株进行比较。方法利用RTPCR反应,从感染了CTN-1-V病毒的小鼠脑内获得GPcDNA的部分片段,并进行序列测定。结果三个代次的CTN-1-V病毒株GPcDNA序列的690个核苷酸与代表性狂犬病毒GP核苷酸相应序列同源性为83.2%～96.8%,氨基酸序列同源性为90.0%～97.4%。CTN1V株三代之间的核苷酸序列几乎相同。结论CTN1V株在传代过程中GP基因结构基本稳定,与中国流行的代表性街毒株的同源性高于aG株和PV株。

GP Gene Sequencing of Three Passages of Current Rabies Vaccine Strain CTN-1-V in China

Objective To analyze the sequence of glycoprotein(GP) gene of three passages of rabies vaccine strain CTN-1-V in China and compare with that of street virus strain.Methods The GP cDNA fragments were amplified from the murine brain infected with CTN-1-V strain by RT-PCR then sequenced.Results The homologies of nucleic acid and amino acid sequences of three passages of CTN-1-V strain to those of street strain were 83.2%-96.8% and 90.0%-97.4% respectively.The nucleic acid sequences of three passages of CTN-1-V strains were basically identical.Conclusion The structure of GP gene of CTN-1-V strain was bascially stable during passage,and the homology of CTN-1-V to street strain was higher than those of aG and PV strains.