不同骨密度测量方法在补钙剂评价中的应用

目的:评价某补钙为主保健食品增加大鼠骨密度的功效,比较双能X线吸收法(DEXA)与阿基米德定律法在骨密度测量中的应用。方法:参照《保健食品检验与技术评价规范》(2003版")增加骨密度功能检验方法"方案一,将45只断乳Sprague-Dawley大鼠按体质量随机分为碳酸钙对照组、低钙对照组和保健品低、中、高剂量组(0.17、0.33、1.00g/kg),对某保健食品进行评价。结果:保健品高剂量组及碳酸钙对照组大鼠在钙表观吸收率、质量增加量、骨密度及骨钙含量上明显高于低钙对照组(P<0.05)。除骨密度外,保健品高剂量组同碳酸钙对照组相比各指标差异不显著;DEXA法与阿基米德定律法所测骨密度结果相关性较好。结论:该保健食品对大鼠有增加骨密度作用;DEXA法与阿基米德定律法均可用于动物实验中骨密度的测定。

Application of Different Bone Mineral Density Measurements in the Assessment of Calcium Supplement

Objective: To assess the enhancing effect of a commercially available calcium supplement on bone mineral density in rats and to compare the application of dual energy X-ray absorptiometry(DEXA) and Archimedes,principle bone mineral density.Methods: According to Protocol 1 from Method for Testing the Function of Increasing Bone Density,,2003 Technical Standards for Testing and Assessing of Health Food,45 weaned SD rats were divided at random into calcium carbonate control group,low calcium control group,and high-dose,medium-dose and low-dose calcium supplement groups(administered at doses of 1.00,0.33 g/kg and 0.17 g/kg,respectively).Results: The high-dose group showed a significant increase in apparent calcium absorption rate,body weight gain,and bone mineral density and calcium content when compared with the low-dose group(P < 0.05).All indexes investigated except bone mineral density indicated no significant difference between the high-dose and calcium carbonate control groups.There was a good correlation between the results of bone mineral density determination by the DEXA method and Archimedes,principle.Conclusions: The calcium supplement has an enhancing effect on bone mineral density in rats.Moreover,the DEXA method and Archimedes,principle are both applicable for the determination of bone mineral density.