Delayed effects of environmentally relevant concentrations of 3,3',4,4'-tetrachlorobiphenyl (PCB-77) and non-polar sediment extracts detected in the prolonged-FETAX

In the prolonged-FETAX (prolonged-Frog Embryo Teratogenic Assay-Xenopus) tadpoles are allowed to develop until metamorphosis after an initial 4 day early life-stage exposure (FETAX). PCB 77 (3,4,3',4'-tetrachlorobiphenyl) and sediment extracts were used in the presented experiments. Concentrations of PCB 77 (0.03 nM=8.55 ng/L; 3 nM=855 ng/L; 300 nM=85.5 microg/L, 30,000 nM=8.55 mg/L) were derived on the basis of dioxin-like toxic equivalency concentrations known to be present in pore-water. The results were compared to those obtained with the standard FETAX. In the prolonged-FETAX the percentage of animals passing metamorphosis within 115 days was significantly decreased in the groups exposed to 300 or 30,000 nM PCB 77. Significantly increased percentages of tadpoles were halted in thyroid hormone dependent early metamorphic NF stages