| 种子扩大培养过程对类人胶原蛋白规模化生产的影响 |
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| 引用本文： | 薛文娇,范代娣.种子扩大培养过程对类人胶原蛋白规模化生产的影响[J].化学与生物工程,2011,28(6):29-32. |
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| 作者姓名： | 薛文娇 范代娣 |
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| 作者单位： | 1. 陕西省微生物研究所,陕西西安,710043
2. 西北大学化工学院陕西省可降解生物医药材料重点实验室,陕西西安,710069 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 为了建立最优的类人胶原蛋白(HLC)的种子扩大培养过程，考察了三级种子培养过程中不同移种阶段和不同种子培养基对发酵过程的影响。结果表明，在对数期后期移种，HLC产率最高；当种子培养基中葡萄糖浓度为20 g·L-1时，其后发酵过程所需的培养时间较短，HLC表达量较高，HLC平均产率最高，达到0.518 g·L-1·h-1...
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| 关 键 词： | 种子扩大培养过程 移种阶段 种子培养基 重组大肠杆菌 类人胶原蛋白 |
Effects of Inoculum Preparation Process on the Pilot Productionof Human-like Collagen |
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| XUE Wen-jiao,FAN Dai-di.Effects of Inoculum Preparation Process on the Pilot Productionof Human-like Collagen[J].Chemistry & Bioengineering,2011,28(6):29-32. |
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| Authors: | XUE Wen-jiao FAN Dai-di |
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| Affiliation: | 1.Shanxi Institute of Microbiology,Xi′an 710043,China;2.Shanxi Key Laboratory of Degradable Biomedical Materials,School of Chemical Engineering,Northwest University,Xi′an 710069,China) |
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| Abstract: | To optimize the inoculum preparation process for production of human-like collage(HLC) by E.coli fermentation,the third inoculum transfer stage and medium were determined.The results showed that HLC productivity was the highest when the third inoculum was transferred at lately exponential stage;in addition,when the glucose concentration was 20 g·L-1,the following fermentation time was the shortest and the final HLC expression quantity was the highest,and thus the HLC average productivity was the highest(0.518 g·L-1·h-1). |
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| Keywords: | inoculum preparation process inoculum transfer stage inoculum medium recombinant Escherichia coli human-like collagen |
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