| 深海微生物絮凝剂HBF-3的剂型研究 |
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| 引用本文： | 赵妮,印文,王阶平,邵宗泽,喻子牛,何进. 深海微生物絮凝剂HBF-3的剂型研究[J]. 化学与生物工程, 2011, 28(6): 33-35. DOI: 10.3969/j.issn.1672-5425.2011.06.009 |
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| 作者姓名： | 赵妮 印文 王阶平 邵宗泽 喻子牛 何进 |
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| 作者单位： | 1. 华中农业大学生命科学技术学院,湖北武汉,430070
2. 国家海洋局第三海洋研究所,福建厦门,361000 |
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| 基金项目： | 国际海底区域研究开发“十一五”项目 |
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| 摘 要： | 对深海微生物絮凝剂HBF-3的剂型进行了研究。结果表明，以5％蔗糖为保护剂，采用冷冻干燥技术将发酵液制成固体剂型，冻干的HBF-3能够迅速复溶于水，絮凝活性为82.4％，维持了较高的絮凝活性；采用旋转蒸发浓缩将发酵液制成液体剂型，HBF-3浓缩液按浓缩倍数稀释后的絮凝活性与初始发酵液相比，变化不大；在液体剂型HBF-3...
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| 关 键 词： | 微生物絮凝剂 剂型 冷冻干燥 旋转蒸发浓缩 絮凝活性 |
Study on Formulation of the Deep-Sea Microbial Flocculant HBF-3 |
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| ZHAO Ni,YIN Wen,WANG Jie-ping,SHAO Zong-ze,YU Zi-niu,HE Jin. Study on Formulation of the Deep-Sea Microbial Flocculant HBF-3[J]. Chemistry & Bioengineering, 2011, 28(6): 33-35. DOI: 10.3969/j.issn.1672-5425.2011.06.009 |
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| Authors: | ZHAO Ni YIN Wen WANG Jie-ping SHAO Zong-ze YU Zi-niu HE Jin |
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| Affiliation: | 1(1.College of Life Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;2.Third Institute of Oceanography of State Oceanic Administration,Xiamen 361000,China) |
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| Abstract: | The formulation of the novel microbial flocculant HBF-3 was studied in this paper.The supernatant of the fermentative culture was made into solid formulation by freeze-drying with 5% sucrose as a cryoprotectant.The lyophilized power of HBF-3 could be easily rehydrated and therefore kept a flocculating activity as high as 82.4%.The liquid formulation was obtained through straightforward rotary evaporation process,which could mainly maintain the flocculating activity unchanged when diluting the concentrate to original concentration.The results also demonstrated that HBF-3 was thermostable.More importantly,the liquid formulation HBF-3 added 1.0 g·L-1 sorbic acid potassium maintained above 90% flocculating activity even one month later when stored at 4 ℃,28 ℃,37 ℃,and 50 ℃,respectively. |
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| Keywords: | microbial flocculant formulation freeze-drying rotary evaporation flocculating activity |
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