獾油、貂油、貉油的脂肪酸组成及其抗氧化、抑菌性能研究

分别以獾、貂、貉皮下脂肪为原料,提取獾油、貂油、貉油。用气质联用仪分析了獾油、貂油、貉油的脂肪酸组成,并分别对其进行了DPPH自由基清除能力的测定。采用倾注法测定了獾油、貂油、貉油对大肠杆菌、鼠伤寒沙门氏菌、金黄色葡萄球菌的抑制性能。结果表明:獾油、貂油、貉油的主要脂肪酸组成均为油酸、亚油酸和棕榈酸,不饱和脂肪酸含量分别为76.296%、76.515%、78.908%;獾油、貂油、貉油清除DPPH自由基的IC50分别为0.315、0.074、0.081 g/m L,即对DPPH自由基清除能力貂油略优于貉油,明显优于獾油;獾油、貂油、貉油对大肠杆菌、鼠伤寒沙门氏菌、金黄色葡萄球菌均无抑制作用。

Fatty acid composition, antioxidation and antibacterial properties of Meles meles oil, mink oil and raccoon dog oil

The Meles meles oil, mink oil and raccoon dog oil were extracted from the subcutaneous fat of Meles meles, mink and raccoon respectively.The fatty acid compositions of Meles meles oil, mink oil and raccoon dog oil were analyzed by gas chromatography-mass spectrometry (GC-MS), and their antioxidant capacities in vitro were respectively tested by 1,1-diphenyl-2- picrylhydrazyl (DPPH) free radicals scavenging capacity.The pouring method was used to determine the antibacterial properties of the three oils against E. coli, Salmonella and Staphylococcus aureus.The results showed that the main fatty acid compositions of Meles meles oil, mink oil and raccoon dog oil were oleic acid, linoleic acid and palmitic acid, and their unsaturated fatty acids contents were 76.296%, 76.515% and 78.908%, respectively.The IC50 for scanvenging DPPH free radicals of Meles meles oil, mink oil, and raccoon dog oil were 0.315, 0.074 g/mL and 0.081 g/mL, respectively. That was, mink oil was slightly better than raccoon dog oil in the scanvenging DPPH free radicals, and was significantly better than Meles meles oil. Meles meles oil, mink oil, and raccoon dog oil had no antibacterial effects on E. coli, Salmonella, and Staphylococcus aureus.