基于斑马鱼胚胎急性毒性测试预测食用油毒性可行性研究

探讨利用斑马鱼胚胎预测食用油毒性的可行性。用乙腈和正己烷联合提取采自我国台湾地区的7个不同品质的猪油样本，并用甲醇溶解，然后用斑马鱼胚胎测试提取物的毒性，最后用非靶标高分辨率复合四极杆质谱仪偶联超高液相色谱/质谱系统进一步分析低毒、中毒、高毒代表性样本中的化学组成，结合ChemSpider、CT-link和Vega软件分析预测有毒物质的结构和功能。结果表明，不同品质的猪油样本毒性差异大，高毒性猪油的特征性质荷比信号相对应的化学成分为脂肪酸的氧化物，是样本的潜在有毒成分。斑马鱼胚胎急性毒性测试可准确地预测猪油品质，该方法为评估食用油安全提供了一个新的思路。

A feasibility study on predicting the toxicity of edible oils using zebrafish embryos

The feasibility of predicting the toxicity of edible oils using zebrafish embryos was investigated. Seven lards with different qualities were collected from Taiwan, China and were extracted with acetonitrile and n-hexane, and then dissolved with methanol. The acute toxicity of the lard extracts was assessed using zebrafish embryos. The potential toxic component in the representative low, moderate, and high toxicity samples were further analyzed using non-target high-resolution Hybrid Quadrupole-Orbitrap mass spectrometer coupled on-line to a UHPLC/MS system. The structure and function of the potential toxic components in lard sample were predicted using ChemSpider, CT-link and Vega softwares. The results showed that the acute toxicity of the lard with different qualities were very different, and the potential toxic compounds in the high toxicity lard were identified to be oxidized fatty acid. Zebrafish embryo acute toxicity test could well predict the quality of different lards.The method provided a new idea to assess the safety of edible oils.