桐油酶水解制备桐酸的工艺研究

为探索利用桐油酶水解制备桐酸的合理工艺,采用单因素实验考察了水解温度、水添加量和酶添加量对桐油水解率的影响,在此基础上以桐油水解率和α-桐酸保留率为指标,运用响应面法对桐油酶水解工艺条件进行优化。结果表明:桐油酶水解制备桐酸的最佳工艺条件为水解温度45℃、水添加量22%、酶添加量0.5%,在此条件下桐油水解率为90.15%,α-桐酸保留率为98.26%。

Preparation of eleostearic acid by enzymatic hydrolysis of tung oil

In order to explore the reasonable process for preparing eleostearic acid by enzymatic hydrolysis of tung oil,the effects of hydrolysis temperature,amount of water and amount of enzyme on hydrolysis rate of tung oil were studied by single factor experiment. Then with hydrolysis rate of tung oil and α-eleostearic acid retention rate as indexes,the enzymatic hydrolysis conditions of tung oil were optimized using response surface methodology. The results showed that the optimal process parameters for preparing eleostearic acid by enzymatic hydrolysis of tung oil were obtained as follows: hydrolysis temperature45 ℃,amount of water 22% and amount of enzyme 0. 5%. Under these conditions,the hydrolysis rate of tung oil was 90. 15% and the α-eleostearic acid retention rate was 98. 26%.