银杏叶中黄酮类化合物的提取和分离

对银杏叶中黄酮类化合物的乙醇提取工艺和膜分离纯化工艺进行研究,并优化工艺条件。结果表明:采用70%乙醇溶液在料液比1∶10、80℃条件下提取2 h,然后采用截留相对分子质量依次为30 000、10 000、5 000的陶瓷膜组成梯度膜装置,在压力0.25 MPa、温度30℃、时间120 min条件下进行分离纯化,黄酮透过率为94.22%,产品中黄酮含量为45.60%。

Extraction and separation of flavonoids from Ginkgo biloba leaves

The ethanol extraction and membrane separation process of flavonoids from Ginkgo biloba leaves were studied,and the process conditions were optimized as follows: extracting Ginkgo biloba leaves for2 h at 80 ℃ and ratio of material to solvent 1 ∶ 10 with 70% ethanol,then separated and purified by a gradient membrane device composed of ceramic membranes with relative molecular weights of 30 000,10 000 and 5 000 under the conditions of the pressure 0. 25 MPa,temperature 30 ℃ and time 120 min,and the flavonoid transmission rate was 94. 22%,and the content of flavonoids in the product was45. 60% after membrane separation.