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白腐菌Panus conchatus降解稻草木素机理研究──(一)低分子木素降解产物各组分的分离及特征
引用本文:付时雨,余惠生,文湘辉,黄秀瑜.白腐菌Panus conchatus降解稻草木素机理研究──(一)低分子木素降解产物各组分的分离及特征[J].纤维素科学与技术,1997(1).
作者姓名:付时雨  余惠生  文湘辉  黄秀瑜
作者单位:中国科学院广州化学研究所纤维素化学开放研究实验室!广州,510650,中国科学院广州化学研究所纤维素化学开放研究实验室!广州,510650,中国科学院广州化学研究所纤维素化学开放研究实验室!广州,510650,中国科学院广州化学研究所纤维素化学开放研究实验室!广州,510650
基金项目:国家自然科学基金,广东省自然科学基金
摘    要:经白腐菌Panusconchatuss发酵15天的稻草用乙酸乙酯进行抽提可有效分离出生物作用后的低分子量木煮降解产物。利用这些低分子降解产物在酸碱度不同的介质以及不同的有机溶剂中的溶解度不同进一步分离、分级和提纯,获得酚类(LP),芳香酸类(LA1—4)以及中性物质三个组份。我们发现LP与LA是降解产物中的主要组份,它们的得率分别为51.1%和34.4%。如此高的LP得率是白腐菌P.conchatus降解稻草木素的重要特征。本文还利用红外光谱和核磁共振技术对LP和LA中最多的一个组份LA—1的可能化学结构特征进行剖析。

关 键 词:白腐菌  Panusconchatus  木素生物降解  稻草

Investigations related to biodegradation of lignin in rice straw by Panus conchatus──Ⅰ.Isolation and characterization of low-molecular weight fractions of the lignin degradation products
Fu Shigu, Yu Huisheng, Wen Xianghui, Huang XiuyuLaboratory of Cellulose and Lignocellulosics,.Investigations related to biodegradation of lignin in rice straw by Panus conchatus──Ⅰ.Isolation and characterization of low-molecular weight fractions of the lignin degradation products[J].Journal of Cellulose Science and Technology,1997(1).
Authors:Fu Shigu  Yu Huisheng  Wen Xianghui  Huang XiuyuLaboratory of Cellulose and Lignocellulosics  
Abstract:Low-molecular weight lignin degradative product was efficiently isolated byextraction of decayed rice straw with ethyl acetate. The low-molecular weight productsof the lignin degradation were further fractionated into phenolic (LP), aromatic acid(LA) and neutral (NS) fractions by means of so1ubi1ity in different basic solutions andorganic solvents. It was found that LP and LA were main ftactions and the yield of LPand LA was 51. 1% and 34. 4% respectively. High yield of LP was an important featherof lignin biodegradation by P. conchatus. LP and LA ftactions were further characterizedby IR and 1H-NMR spectroscopy.
Keywords:white-rot fungus  P  conchatus  rice straw  lignin biodegradation  
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