Supercritical carbon dioxide extraction of lipids from Pythium irregulare |
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Authors: | Terry H. Walker Hank D. Cochran Greg J. Hulbert |
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Affiliation: | (1) the Department of Biological and Agricultural Engineering, Louisiana State University, 149 E.B. Doran Bldg, 70803 Baton Rouge, LA;(2) Chemical Technology Division, Oak Ridge National Laboratory, 37831 Oak Ridge, Tennessee;(3) Department of Food Science and Technology, University of Tennessee, 37901 Knoxville, Tennessee |
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Abstract: | Lipids that contain polyunsaturated fatty acids (PUFA) have therapeutic value. PUFA, however, degrade in high-temperature, oxygen-rich conditions typical of conventional hot solvent-extraction and distillation methods. Supercritical CO2 extraction was chosen as an alternative method to recover these valuable compounds from the lower fungus, Pythium irregulare. Freeze-dried biomass was subjected to an aqueous phase and placed into a flow-through extraction apparatus. Extraction of oil from this biomass showed some success for moisture contents as high as 30% (wet basis). The addition of a novel CO2-philic surfactant to the wet biomass with moisture contents as high as 95% (wet basis) increased the extraction rate of fungal oil by more than an order of magnitude. For tests with extraction times of 5 to 6 h, data for the diffusion-controlled region were modeled with an analytical solution to Fick’s second law. Equilibrium data were also obtained for the fungal oil at two isotherms (40 and 60°C) over a pressure range of 13.7 to 27.5 MPa. |
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Keywords: | Extraction kinetics fungi phase equilibria polyunsaturated fatty acids Pythium irregulare supercritical fluid extraction supercritical carbon dioxide surfactant |
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