黑蒜对溃疡性结肠炎BALB/c小鼠炎症反应的影响

目的：通过葡聚糖硫酸钠（dextran sulfate sodium，DSS）诱导的BALB/c小鼠溃疡性结肠炎模型，研究黑蒜对溃疡性结肠炎小鼠炎症反应的影响。方法：小鼠自由饮水摄入质量分数4% DSS建立溃疡性结肠炎模型；分别灌胃不同剂量黑蒜水提取液；考察小鼠体质量变化、疾病活动指数（disease activity index，DAI）、病理组织学评分；苏木精-伊红染色观察小鼠结肠组织病理变化，酶联免疫吸附测定法检测小鼠血清肿瘤坏死因子α（tumor necrosis factor α，TNF-α）、白细胞介素6（interleukin 6，IL-6）、IL-1β质量浓度，免疫印迹法检测小鼠结肠组织中TNF-α的表达水平。结果：高、中、低剂量的黑蒜均缓解了DSS引起的小鼠体质量下降；与模型组相比，黑蒜高、低剂量组小鼠DAI评分显著降低（P＜0.05），黑蒜高、中剂量组小鼠病理组织学评分、IL-6、IL-1β和TNF-α质量浓度极显著降低（P＜0.01）；结论：黑蒜具有降低BALB/c小鼠溃疡性结肠炎炎症反应的作用。

Effect of Black Garlic on Inflammation in BALB/c Mice with Ulcerative Colitis

Objective: To study the effect of black garlic on inflammatory response in BALB/c mice with ulcerative colitis (UC) induced by dextran sulfate sodium (DSS). Methods: The animal model was established by allowing them to have free access to 4% DSS. Afterwards, the mice were gavaged with a water extract of black garlic at different doses. The changes in the body mass of mice were determined as well as disease activity index (DAI) and histological score, and hematoxylin-eosin staining was used to evaluate the pathological changes in the colon. The serum concentrations of tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β) were determined by enzyme-linked immunosorbent assay. Meanwhile, TNF-α expression in the colon was evaluated by Western blot. Results: black garlic at all three doses alleviated the loss of body mass induced by DSS in mice. Compared with the model control group, black garlic at the high and low doses significantly decreased DAI (P < 0.05) and the histopathological score and the levels of IL-6, IL-1β and TNF-α in mice in the high and medium dose black garlic groups were significantly lower (P < 0.01). Conclusion: Black garlic can attenuate the inflammatory reaction in BALB/c mice with UC.