超高效液相色谱测定冷冻研磨甘薯植株中酚酸及其分布

建立超高效液相色谱法分析13种甘薯品种中不同植株酚酸的含量与分布.结果 表明,在萃取溶剂中加入0.2％亚硫酸氢钠可以提高咖啡酸和咖啡酰奎宁酸类成分的回收率和稳定性.不同的样品预处理方法比较发现,冷冻研磨获得的酚酸含量最高,其次是冻干、烘干、晒干和直接匀浆.方法 快速、稳定、灵敏度高,加标回收率为99.5％～103.1％...

Determination of Phenolic Acids and Their Distribution in Freeze-Ground Aerial Parts of Different Sweetpotato Varieties by Ultra-High Performance Liquid Chromatography

In this study, an ultra-high performance liquid chromatography (UPLC) method was established to analyze the distribution of phenolic acids in aerial parts of 13 sweetpotato varieties. The results showed that addition of 0.2% sodium hydrogen sulfite to the extraction solvent could improve the recovery and stability of caffeic acid (CA) and caffeoylquinic acids (CQAs). Among the tested pretreatment methods, freeze grinding resulted in the highest content of phenolic acids, followed by freeze drying, oven drying, sun drying and homogenization. The UPLC method was fast, stable and highly sensitive with spiked recoveries of 99.5%?103.1%, and precision (expressed as relative standard deviation, RSD) of 0.3%?1.2%. The limit of detection (LOD) and the limit of quantification (LOQ) were 3.6?21.4 and 12.1?71.3 ng/mL, respectively. The contents of total CA and CQAs including CA, 3-CQA, 4-CQA, 5-CQA, 3,4-diCQA, 3,5-diCQA, 4,5-diCQA and 3,4,5-triCQA in the leaves, petioles and stems of sweatpotato ranged from 18.0?44.8, 0.7?11.9, and 0.3?9.7 g/kg dry sample, respectively. The contents of phenolic acids in different sweetpotato varieties were obviously different from each other, with dicaffeoylquinic acids (diCQAs) being the most abundant phenolic acid in all varieties. Moreover, diCQAs were the most abundant in the leaves among the three parts.