微波等离子体原子发射光谱测定啤酒中的常量和微量金属元素

建立微波等离子体原子发射光谱（microwave plasma-atomic emission spectroscopy，MP-AES）测定啤酒中4?种常量金属元素（Na、Mg、K、Ca）和10?种微量金属元素（Al、Mn、Fe、Ni、Cu、Zn、Sr、Cd、Ba、Pb）的分析方法。在密闭的消解罐内采用质量分数65% HNO3溶液对啤酒样品进行微波消解，选择分析元素的波长并结合快速线性干扰校正技术校正光谱干扰，通过加入内标元素校正基体效应，采用电感耦合等离子体质谱（inductively coupled plasma-mass spectrometry，ICP-MS）法对相同的样品溶液进行分析比较验证方法的准确性。在优化条件下，分析元素的检出限在0.13～230?μg/L之间，加标回收率为95.6%～104%，相对标准偏差为1.8%～4.0%。采用MP-AES成功测定啤酒样品中的常量和微量金属元素，并与ICP-MS的测定结果基本一致。方法具有简单快速、稳定性好、运行成本低、准确性好的特点，适合于啤酒中多种金属元素的高通量分析。

Determination of Major and Trace Metal Elements in Beer by Microwave Plasma-Atomic Emission Spectroscopy

An analytical method was established for the determination of four major metal elements (Na, Mg, K, and Ca) and ten trace metal elements (Al, Mn, Fe, Ni, Cu, Zn, Sr, Cd, Ba, and Pb) in beer by microwave plasma-atomic emission spectroscopy (MP-AES). The samples were microwave digested using HNO3 in closed digestion vessels. The spectral interferences were corrected by selecting a suitable wavelength as well as using fast linear interference correction (FLIC). The matrix effect was corrected by using internal standard elements. Inductively coupled plasma-mass spectrometry (ICP-MS) was used to analyze the same sample solution to demonstrate the accuracy of the method. Under the optimized conditions, the limits of detection (LODs) for the analytes were in the range of 0.13–230 μg/L. The spiked recoveries ranged from 95.6% to 104% with relative standard deviations (RSDs) of 1.8%–4.0%. All the major and trace metal elements were successfully determined in beer samples by the MP-AES method and the results obtained were identical to those obtained by ICP-MS. The method has the characteristics of simple and fast operation, good stability, low running cost and good accuracy, and is suitable for high throughput analysis of multiple metal elements in beer.