核桃低聚肽对大鼠胃黏膜损伤的保护作用

本实验通过建立无水乙醇诱导的大鼠胃黏膜损伤模型,探讨核桃低聚肽(walnut oligopeptides,WOPs)对胃黏膜损伤的保护作用及可能机制.雄性SD大鼠根据体质量被随机分为8组(n=10):正常对照组、模型对照组、乳清蛋白组(440 mg/kg mb)、奥美拉唑组(20 mg/kg mb)、3个WOPs剂量组...

Protective Effect of Walnut Oligopeptides against Gastric Mucosal Injury of Rats

The study investigated the protective effect of walnut oligopeptides (WOPs) against absolute ethanol-induced gastric injury, and it also explored the possible underlying mechanism. Male Sprague Dawley (SD) rats were randomly divided into eight groups based on body mas (n = 10), including normal, model, whey protein (440 mg/kg mb), omeprazole (20 mg/kg mb), low-, medium- and high-dose WOP (220, 440, 880 mg/kg mb, respectively) and WOPs (440 mg/kg mb) + bovine collagen oligopeptides (BCOPs, 1.5 g/kg mb) groups. After oral gavage for 30 days, the rats were administered with 5 mL/kg mb of absolute ethanol to induce gastric ulcer, then the gross lesion of gastric tissue was observed and scored, and the levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gastric mucin (MUC), pepsinogen (PG) and gastrin (GAS) were detected. The results showed that administration with WOPs markedly mitigated the hemorrhagic gastric lesions caused by ethanol in rats, inhibited the increase in serum ALT and AST levels and gastric PGI, PGII and GAS levels, and enhanced MUC biosynthesis. These results indicated that WOPs have obvious protective effects against ethanol-induced gastric mucosal injury in rats mainly by attenuating the gastric mucosal damage directly caused by ethanol, inhibiting the biosynthesis of PG and GAS, and enhancing MUC biosynthesis.