| 铜、碘掺杂碳点过氧化物模拟酶比色探针检测茶叶中痕量Pb(II) |
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| 引用本文： | 程春生,杨德志,李宏,李秋兰,杨亚玲. 铜、碘掺杂碳点过氧化物模拟酶比色探针检测茶叶中痕量Pb(II)[J]. 食品科学, 2021, 42(24): 323-328. DOI: 10.7506/spkx1002-6630-20201214-170 |
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| 作者姓名： | 程春生 杨德志 李宏 李秋兰 杨亚玲 |
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| 作者单位： | （1.昆明理工大学食品科学与工程学院，云南 昆明 650500；2.昆明理工大学生命科学与技术学院，云南 昆明 650500；3.云南省农业科学院农产品加工研究所，云南 昆明 650032） |
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| 基金项目： | 云南省科技人才和平台计划-技术创新人才培养对象项目（202005AD160015）；云南省科技创新及成果转化专项-云南省农科院科技专项（202002AE320007-03） |
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| 摘 要： | 基于Pb(II)对铜、碘掺杂碳点模拟酶的活性调节，建立简单、灵敏测定茶叶中Pb(II)的比色探针新方法。铜、碘掺杂碳点的动力学分析结果表明：其模拟酶催化参数符合典型的米氏方程模型。Pb(II)吸附于铜、碘掺杂碳点表面后，可促进底物3,3’,5,5’-四甲基联苯胺（3,3’,5,5’-tetramethylbenzidine，TMB）的氧化。Pb(II)在0.27～27.02?mg/L范围内呈良好的线性关系。将该方法用于Pb(II)含量的测定，其检出限可达到34.3?μg/L，实际茶叶样品的加标回收率在92.41%～101.85%范围内，满足GB?5009.12—2017《食品中铅的测定》中的检测要求。该方法具有简便、稳定、特异性强等特点，其检出限也低于国家规定的食品中Pb(II)含量限值。
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| 关 键 词： | 铅；铜、碘掺杂碳点；过氧化物模拟酶；比色探针；茶叶 |
A Colorimetric Probe for Determination of Trace Pb(II) in Tea Based on Peroxidase-like Activity of Carbon Dots Co-doped with Copper and Iodine |
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| CHENG Chunsheng,YANG Dezhi,LI Hong,LI Qiulan,YANG Yaling. A Colorimetric Probe for Determination of Trace Pb(II) in Tea Based on Peroxidase-like Activity of Carbon Dots Co-doped with Copper and Iodine[J]. Food Science, 2021, 42(24): 323-328. DOI: 10.7506/spkx1002-6630-20201214-170 |
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| Authors: | CHENG Chunsheng YANG Dezhi LI Hong LI Qiulan YANG Yaling |
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| Affiliation: | (1. Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China; 2. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China; 3. Institute of Agro-products Processing, Yunnan Academy of Agricultural Sciences, Kunming 650032, China) |
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| Abstract: | Based on the regulation of Pb(II) on the enzyme-like activity of carbon dots co-doped with copper and iodine (Cu, I-CDs), a novel, simple and sensitive colorimetric probe was established for the determination of Pb(II) in tea samples. The kinetic parameters of Cu, I-CD peroxidase-like activity were well fitted to the Michaelis-Menten equation. When it was adsorbed on the surface of Cu, I-CDs, Pb(II) could promote the oxidation of the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The concentration of Pb(II) in the range of 0.27–27.02 mg/L showed a good linear relationship with the formation of oxidized TMB (oxTMB). The detection limit of the developed method was 34.3 μg/L, and the spiked recoveries for actual tea samples were in the range of 92.41%–101.85%. These figures of merit meet the requirements of the national standard GB 5009.12-2017. In conclusion, this method is simple, stable, and specific, and its detection limit is lower than the country’s limit for Pb(II) content in foods. |
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| Keywords: | lead carbon dots co-doped with copper and iodine peroxidase mimic colorimetric probe tea, |
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