| 基于智能手机同时检测4种兽药残留的免疫芯片技术 |
| |
| 引用本文： | 吴颖,范丛丛,苏晓娜,沈玉栋,谭庶,钟翠丽,许小炫,曾道平,杨金易. 基于智能手机同时检测4种兽药残留的免疫芯片技术[J]. 食品科学, 2021, 42(2): 278-282. DOI: 10.7506/spkx1002-6630-20190927-334 |
| |
| 作者姓名： | 吴颖 范丛丛 苏晓娜 沈玉栋 谭庶 钟翠丽 许小炫 曾道平 杨金易 |
| |
| 作者单位： | （1.华南农业大学食品学院，广东 广州 510642；2.温氏食品集团股份有限公司，广东 云浮 527499） |
| |
| 基金项目： | “十三五”国家重点研发计划重点专项（2018YFC1602904）；广东省自然科学基金重大培育项目（2018B030314005）；国家自然科学基金面上项目（31871887）；广州市民生科技重大专项（201704020082） |
| |
| 摘 要： | 建立一种基于智能手机同时检测猪尿中莱克多巴胺、盐酸克仑特罗、氯霉素、氟苯尼考及其代谢物氟苯尼考胺多种兽药残留的免疫蛋白芯片方法.以硝酸纤维素膜(NC膜)为固相载体固定4种药物抗原,基于分析物与固相抗原竞争抗体的反应,利用不同质量浓度的药物标准品与芯片显色斑点检测强度作标准曲线,建立智能手机检测方法.该方法对莱克多巴胺、...
|
| 关 键 词： | 免疫芯片 兽药 多残留 智能手机 |
Simultaneous Detection of Four Veterinary Drugs Using Immunochip Assay Based on Smart Phone |
| |
| WU Ying,FAN Congcong,SU Xiaona,SHEN Yudong,TAN Shu,ZHONG Cuili,XU Xiaoxuan,ZENG Daoping,YANG Jinyi. Simultaneous Detection of Four Veterinary Drugs Using Immunochip Assay Based on Smart Phone[J]. Food Science, 2021, 42(2): 278-282. DOI: 10.7506/spkx1002-6630-20190927-334 |
| |
| Authors: | WU Ying FAN Congcong SU Xiaona SHEN Yudong TAN Shu ZHONG Cuili XU Xiaoxuan ZENG Daoping YANG Jinyi |
| |
| Affiliation: | (1. College of Food Science, South China Agricultural University, Guangzhou 510642, China; 2. Wens Food Group Co. Ltd., Yunfu 527499, China) |
| |
| Abstract: | An immunochip based on smart phone for simultaneous detection of four veterinary drugs including ractopamine (RAC), clenbuterol (CL), chloramphenicol (CAP), florfenicol (FF) and its metabolite florfenicol amine (FFA) in pig urine was developed in this study. The immunochip test was conducted based on the competitive reactions with the antibodies on nitrocellulose (NC) membrane. After chromogenic reaction, a calibration curve for the concentration of each veterinary drug versus the intensity of spots on the immunochip was established. The limits of detection (LODs) of the immunochip assay for RAC, CL, CAP, and FF were 0.146, 0.137, 0.035, and 3.73 ng/mL respectively, and the linear working ranges were 0.353–1.017, 0.309–0.897, 0.082–0.249, and 9.424–35.594 ng/mL, respectively. The cross-reactivity rate with FFA was 81.5%. The results determined by this method were consistent with those determined by colloidal gold immunochromatography assay (GICA) and enzyme-linked immunosorbent assay (ELISA). To sum up, this immune chip is a cheap, simple and powerful tool for on-site and high throughput screening of veterinary drug residues. |
| |
| Keywords: | immunochip veterinary drugs simultaneous detection smart phone, |
|
| 点击此处可从《食品科学》浏览原始摘要信息 |
|
点击此处可从《食品科学》下载全文 |
|
