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固载离子液体修饰Fe3O4纳米酶用于H2O2和葡萄糖的检测
引用本文:杨培昕,喻昌木,杨敏,张荣,汤陆扬,付阳洋,彭黔荣. 固载离子液体修饰Fe3O4纳米酶用于H2O2和葡萄糖的检测[J]. 食品科学, 2021, 42(20): 252-259. DOI: 10.7506/spkx1002-6630-20200924-296
作者姓名:杨培昕  喻昌木  杨敏  张荣  汤陆扬  付阳洋  彭黔荣
作者单位:(1.贵州大学药学院,贵州 贵阳 550025;2.贵州大学化学与化工学院,贵州 贵阳 550025;3.贵州中烟工业有限责任公司技术中心,贵州 贵阳 550009)
基金项目:国家自然科学基金地区科学基金项目(21562014);贵州中烟科技项目(黔烟工技[2015]8号)
摘    要:研究SiO2固载离子液体修饰的磁性纳米粒子(Fe3O4@SiO2@IL)制备,及其H2O2酶活性测试。结果表明,Fe3O4@SiO2@IL具有比辣根过氧化物酶更高的催化活性,并能催化H2O2与过氧化物酶底物3,3’,5,5’-四甲基联苯胺形成蓝色产物。因此,开发H2O2比色检测方法,线性范围为4~100 μmol/L,检出限为0.698 μmol/L。该方法还可用于葡萄糖的高灵敏度和选择性检测,线性范围为8~200 μmol/L,检出限为2.39 μmol/L。将该方法用于检测实际样品(如牛奶)中的H2O2,加标回收率为94.0%~107.8%,相对标准偏差为2.24%~3.12%,准确度和精密度较高。具有过氧化物酶活性的Fe3O4@SiO2@IL纳米材料在临床诊断、制药、食品研究等领域具有潜在的应用价值。

关 键 词:比色传感器  离子液体  纳米酶  H2O2  葡萄糖  食品检测,

Immobilized Ionic Liquid Modified Fe3O4 Nanoenzyme for the Detection of Hydrogen Peroxide and Glucose in Foods
YANG Peixin,YU Changmu,YANG Min,ZHANG Rong,TANG Luyang,FU Yangyang,PENG Qianrong. Immobilized Ionic Liquid Modified Fe3O4 Nanoenzyme for the Detection of Hydrogen Peroxide and Glucose in Foods[J]. Food Science, 2021, 42(20): 252-259. DOI: 10.7506/spkx1002-6630-20200924-296
Authors:YANG Peixin  YU Changmu  YANG Min  ZHANG Rong  TANG Luyang  FU Yangyang  PENG Qianrong
Affiliation:(1. School of Pharmaceutical Sciences, Guizhou University, Guiyang 550025, China;2. School of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550025, China;3. Technology Center of China Tobacco Guizhou Industrial Co. Ltd., Guiyang 550009, China)
Abstract:Silica-immobilized ionic liquid modified magnetic nanoparticles (Fe3O4@SiO2@IL) were prepared and applied as a peroxidase mimic for the detection of H2O2. The results showed that Fe3O4@SiO2@IL had higher catalytic activity than horseradish peroxidase (HRP), and could catalyze the coupling of H2O2 with the peroxidase substrate 3,3’,5,5’-tetramethyl aniline (TMB) to form a blue product. On the basis of this phenomenon, a colorimetric method for H2O2 detection was developed, with a linear range of 4–100 μmol/L and a detection limit of 0.698 μmol/L. The method was also available to sensitively and selectively detect glucose, with a linear range of 8–200 μmol/L and a detection limit of 2.39 μmol/L. The recoveries for this method when applied to detect H2O2 in milk samples ranged from 94.0% to 107.8%, with relative standard deviation (RSD) values of 2.24%–3.12%, showing high accuracy and precision. Therefore, Fe3O4@SiO2@IL nanomaterials with peroxidase mimic activity can be a promising candidate for application in clinical diagnosis, pharmaceutical, food and other fields.
Keywords:colorimetric sensor   ionic liquid   nanoenzyme   hydrogen peroxide   glucose   food detection,
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