魔芋甘露寡糖对酸奶免疫调节活性的影响

益生元用于发酵乳制品可改善其质构特性和感官品质,同时强化其健康功效.本研究采用环磷酰胺(cyclophosphomide,CTX)诱导BALB/C小鼠建立免疫抑制模型,评价功能性低聚糖类益生元——魔芋甘露寡糖(konjac mannanoligosaccharides,KMOS)的添加对于酸奶免疫调节活性的影响.结果表...

Effects of Konjac Mannanoligosaccharide Fortification on Immunomodulatory Activity of Yogurt

Prebiotics can be used in fermented dairy products for improved sensory and textural properties, as well as enhanced health beneficial effects. In this study, the immunomodulatory effects of yogurt fortified with konjac mannanoligosaccharides (KMOS), a new type of functional oligosaccharides, were investigated in immunosuppressed BALB/C mice induced by cyclophosphamide (CTX). The results suggested that addition of KMOS enhanced the ability of yogurt to attenuate CTX-induced immunosuppression. Yogurt added with 0.5% (based on the weight of fresh milk) KMOS significantly improved the basic physiological state, the phagocytic activity of peritoneal macrophages, the proliferation rate of splenic lymphocytes, serum immunoglobulin concentration, and the release of inflammatory cytokines from peritoneal macrophages. Moreover, KMOS-fortified yogurt increased the relative abundance of non-dominant flora and the quantity of intestinal probiotics, and simultaneously suppressed the proliferation of Clostridium in the colon of immunosuppressed mice, thereby modulating the composition and structure of intestinal microbiota. These findings will provide useful information for the development of novel fermented milk products fortified with prebiotic oligosaccharides.